Biolegend, 332303, PE anti-human CD352 (NTB-A) Antibody, 25tests

NTBA (NK-, T-, and B-cell Antigen) is a 60 kD type I transmembrane glycoprotein. It is a member of Ig superfamily belonging to CD2/CD150 subfamily, also known as SLAMF6 (SLAM family member 6), LY108, TCOM, or SF2000. This antigen is expressed on NK cells, T cells (upregulated upon activation), and B cells. NTBA is its own ligand. The homophilic interaction of NTBA is involved in the induction of NK cytotoxicity, CD28 independent T cell costimulation, triggering Th1 cytokines production, and regulation of B cell tolerance. It may play a role in the regulation of autoimmune diseases.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant human CD352 protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC- Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_2890763 (BioLegend Cat. No. 332303) AB_2890763 (BioLegend Cat. No. 332304)
Structure: Type I transmembrane tyrosine-phosphorylated glycoprotein, CD2/CD150 subfamily, 60 kD
Distribution: NK cells, T cells, B cells
Function: CD28 independent T cell costimulation, induction of NK cell cytotoxicity, regulation of B cell tolerance
Ligand/Receptor: NTBA
Antigen References: 1. Yigit B, et al. 2019. Cancer Immunol. Res. 10.1158/2326-6066 2. Wu N, et al. 2016. Nature Immunol. 17, 387-396 3. Radomir L, et al. 2017. J. Immunol. 199(8) 2745-2757
Gene ID: 114836
UniProt: View information about CD352 on UniProt.org
Clone: W19035D
Regulatory Status: RUO
Other Names: SLAMF6, LY108, TCOM, SF2000, NTBA
Isotype: Rat IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.