Biolegend, 332406, FITC anti-human CD35 Antibody, 100tests

CD35 is a type I single chain of glycoprotein, also known as C3b/C4b receptor, Complement Receptor type 1 or CR1. Four molecular weight allotypes (160kD, 190kD, 220kD, and 250kD) have been described. CD35 is expressed on granulocytes, monocytes, B cells, erythrocytes, and follicular dendritic cells, as well as subsets of NK and T cells. CD35 binds complement C3b, C4b, or iC3, and iC4, and plays important roles in both innate and adoptive immune response via mediating phagocytosis by granulocytes and monocytes. CD35 has also been reported to inhibit T-cell proliferation.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC- Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)
Product Citations: Mannes M, et al. 2023. J Innate Immun. 412:15. PubMed Wohlgemuth L, et al. 2022. Cells. 11: . PubMed
RRID: AB_2890766 (BioLegend Cat. No. 332405) AB_2890766 (BioLegend Cat. No. 332406)
Distribution: B cells, Dendritic cells, Erythrocytes, Granulocytes, Monocytes, NK cells, T cells
Function: Adhesion, mediate phagocytosis, inhibit T-cell proliferation
Ligand/Receptor: C3b, iC3, C4b, iC4
Cell Type: B cells, Dendritic cells, Erythrocytes, Granulocytes, Monocytes, NK cells, T cells
Biology Area: Cell Biology, Complement, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules
Antigen References: 1. Vorup-Jensen et al. 2018. Front Immunol. 9:2716. 2. Ogembo et al. 2013. Cell Rep. 3(2):371-85.
Gene ID: 1378
UniProt: View information about CD35 on UniProt.org
Clone: 9H3
Regulatory Status: RUO
Other Names: C3b/C4b receptor, complement receptor type 1 (CR1)
Isotype: Mouse IgG1, κ