Biolegend, 333201, Purified anti-human DcR3 Antibody, 25μg

The DcR3 protein is a soluble member of the TNF receptor superfamily containing four TNF receptor domains with an approximate molecular weight of 35 kD. DcR3, also known as Decoy receptor 3, TR6, M68, can be secreted by monocytes and myeloid-derived dendritic cells following exposure to bacterial antigens. DcR3 is overexpressed in tumors including lung and colon cancers, gliomas, and pancreatic cancers and can be detected in the serum of cancer patients. DcR3 can also be found in the cytoplasm and endoplasmic reticulum in some cell types. This protein competes for ligand binding with cell surface receptor and can inhibit their signaling. DcR3 has been shown to bind the ligands FasL, LIGHT, and TNF like molecule 1 (TL1A) to inhibit binding to the cell surface receptors Fas, HVEM and LT-ßR, and death receptor 3 (DR3), respectively.
25μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human DcR3 Fc protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: WB - Quality testedIP, IHC - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5-20 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting1, immunoprecipitation2, and immunohistochemistry2.
Application References(PubMed link indicates BioLegend citation): Hsu TL, et al. 2005. J. Immunol. 175:5135. (WB)Personal communication (IP IHC ELISA Detection)Tai SK, et al. 2012. J. Immunol. 188:2464. PubMed.
Product Citations: Yang CY, et al. 2019. Head Neck. 41:1260. PubMed Tai S, et al. 2012. J Immunol. 188:2464. PubMed Weng S, et al. 2015. Sci Rep. 5: 12769. PubMed
RRID: AB_2207467 (BioLegend Cat. No. 333202)
Structure: Soluble member of the TNF superfamily (6B), contains four TNF-receptor like domains, approximate molecular weight 35 kD.
Distribution: Secreted protein, also found in cytoplasm and endoplasmic reticulum. Expressed by monocytes and myeloid-derived dendritic cells following exposure to bacterial antigens. Overexpressed in tumors including lung and colon cancers, gliomas and pancreatic canc
Function: Competes with ligand binding to surface receptors including FasL binding to Fas, LIGHT binding to HVEM and LT-ßR, and TNF like molecule 1 (TL1A) with death receptor 3 (DR3). Competition inhibits cell-surface receptor signaling.
Interaction: Binds FasL, LIGHT, and TL1A
Cell Type: Monocytes, Dendritic cells
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: 1. Pitti RM, et al. 1998. Nature 396:699. 2. Bai C, et al. 2000. Proc. Natl. Acad. Sci. USA 97:1230. 3. Yu KY, et al. 1999. J. Biol. Chem. 274:13733. 4. Migone TS, et al. 2002. Immunity 16:479.
Gene ID: 8771
UniProt: View information about DcR3 on UniProt.org
Clone: B08-35
Regulatory Status: RUO
Other Names: Decoy receptor 3, TR6, M68
Isotype: Mouse IgG1, κ