Biolegend, 333809, APC anti-human CD68 Antibody, 25tests

CD68 is a 110 kD glycoprotein, also known as macrosialin, belonging to the sialomucin family. It is closely related to the family of acidic, highly glycosylated lysosomal-associated membrane proteins (LAMPs). CD68 is predominately expressed in cytoplasmic granules of monocytes/macrophages, dendritic cells, and granulocytes. It is one of the useful myeloid cell markers. Further studies have shown that CD68 is also expressed by a subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, and endothelial cells. The biological function of CD68 is still unknown.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported application: immunohistochemical staining of frozen tissue sections. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.
Application References(PubMed link indicates BioLegend citation): Doussis IA, et al. 1993. J. Clin. Pathol. 46:334. Davey FR, et al. 1988. J. Clin. Pathol. 41:753. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
Product Citations: Sun L, et al. 2021. Cancer Cell. 39:1361. PubMed Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed Jia J, et al. 2023. Cell Death Dis. 14:156. PubMed Chung JY, et al. 2023. Nat Commun. 14:1794. PubMed Wu J, et al. 2022. Nat Commun. 13:676. PubMed Qin L, et al. 2022. Sci Adv. 8:eabn3774. PubMed Cao B, et al. 2022. Nat Commun. 13:6203. PubMed Patra V, et al. 2019. Br J Dermatol. N/A. PubMed Rahn S, et al. 2019. Oncotarget. 1.508333333. PubMed Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed Teng YY, et al. 2022. Front Bioeng Biotechnol. 10:902894. PubMed Ma L, et al. 2021. Clinical Cancer Research. 27(6):1778-1791. PubMed Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed Obradovic A, et al. 2021. Cell. 184(11):2988-3005.e16. PubMed Walter F, et al. 2020. PLoS One. 15:e0239369. PubMed Chen Y, et al. 2021. Transl Lung Cancer Res. 10:2193. PubMed Rai MA, et al. 2020. Retrovirology. 17:35. PubMed Xiang W, et al. 2018. Nat Commun. 9:2574. PubMed Tamò L, et al. 2021. Int J Mol Sci. 22:00. PubMed Ciccia F, et al. 2014. Rheumatology. 53:104. PubMed Du Q, et al. 2018. J Immunol. 201:533. PubMed Liu W, et al. 2022. J Clin Invest. 132: . PubMed Luvanda MK, et al. 2021. J Fungi (Basel). 7:. PubMed Carlsen T, et al. 2015. J Immunol Methods. 422: 59-71. PubMed
RRID: AB_10567107 (BioLegend Cat. No. 333809) AB_2275735 (BioLegend Cat. No. 333810)
Structure: Sialomucin family, 110 kD
Distribution: Monocytes/macrophages, dendritic cells, granulocytes, subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, endothelial cells
Cell Type: B cells, Dendritic cells, Endothelial cells, Fibroblasts, Granulocytes, Hematopoietic stem and progenitors, Macrophages, Monocytes, T cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Holness CL and Simmons DL. 1993. Blood 81:1607. 2. Gottfried E, et al. 2008. Scand. J. Immunol. 67:453. 3. Hameed A, et al. 1994. Hum. Pathol. 25:872.
Gene ID: 968
UniProt: View information about CD68 on UniProt.org
Clone: Y1/82A
Regulatory Status: RUO
Workshop: VI MR23
Other Names: Macrosialin
Isotype: Mouse IgG2b, κ