Biolegend, 333812, Alexa Fluor® 488 anti-human CD68 Antibody, 100tests

CD68 is a 110 kD glycoprotein, also known as macrosialin, belonging to the sialomucin family. It is closely related to the family of acidic, highly glycosylated lysosomal-associated membrane proteins (LAMPs). CD68 is predominately expressed in cytoplasmic granules of monocytes/macrophages, dendritic cells, and granulocytes. It is one of the useful myeloid cell markers. Further studies have shown that CD68 is also expressed by a subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, and endothelial cells. The biological function of CD68 is still unknown.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported application: immunohistochemical staining of frozen tissue sections. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.
Application References(PubMed link indicates BioLegend citation): Doussis IA, et al. 1993. J. Clin. Pathol. 46:334. Davey FR, et al. 1988. J. Clin. Pathol. 41:753. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
Product Citations: Li M, et al. 2023. Front Immunol. 14:1087923. PubMed Smith MH, et al. 2023. Nat Immunol. . PubMed Zhao P, et al. 2022. MAbs. 14:2107971. PubMed Managò A, et al. 2019. Nat Commun. 10:4116. PubMed McAvoy M, et al. 2020. Front Neurol. 0.905555556. PubMed Ohmatsu H, et al. 2017. Oncoimmunology. 6:e1181237. PubMed Li M, et al. 2020. Nat Commun. 4051:11. PubMed Gage BK, et al. 2020. Cell Stem Cell. 27(2):254-269.e9. PubMed
RRID: AB_2074833 (BioLegend Cat. No. 333811) AB_2074832 (BioLegend Cat. No. 333812)
Structure: Sialomucin family, 110 kD
Distribution: Monocytes/macrophages, dendritic cells, granulocytes, subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, endothelial cells
Cell Type: B cells, Dendritic cells, Endothelial cells, Fibroblasts, Granulocytes, Hematopoietic stem and progenitors, Macrophages, Monocytes, T cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Holness CL and Simmons DL. 1993. Blood 81:1607. 2. Gottfried E, et al. 2008. Scand. J. Immunol. 67:453. 3. Hameed A, et al. 1994. Hum. Pathol. 25:872.
Gene ID: 968
UniProt: View information about CD68 on UniProt.org
Clone: Y1/82A
Regulatory Status: RUO
Workshop: VI MR23
Other Names: Macrosialin
Isotype: Mouse IgG2b, κ