Biolegend, 333824, APC/Fire™ 750 anti-human CD68 Antibody, 100tests

CD68 is a 110 kD glycoprotein, also known as macrosialin, belonging to the sialomucin family. It is closely related to the family of acidic, highly glycosylated lysosomal-associated membrane proteins (LAMPs). CD68 is predominately expressed in cytoplasmic granules of monocytes/macrophages, dendritic cells, and granulocytes. It is one of the useful myeloid cell markers. Further studies have shown that CD68 is also expressed by a subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, and endothelial cells. The biological function of CD68 is still unknown.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. * APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported application: immunohistochemical staining of frozen tissue sections. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.
Application References(PubMed link indicates BioLegend citation): Doussis IA, et al. 1993. J. Clin. Pathol. 46:334. Davey FR, et al. 1988. J. Clin. Pathol. 41:753. Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
Product Citations: Kondo A, et al. 2024. Sci Rep. 16386:14. PubMed
RRID: AB_2800877 (BioLegend Cat. No. 333823) AB_2800878 (BioLegend Cat. No. 333824)
Structure: Sialomucin family, 110 kD
Distribution: Monocytes/macrophages, dendritic cells, granulocytes, subset of hematopoietic progenitors, γ/δ T cells, NK cells, LAK cells, subset of B cells, fibroblasts, endothelial cells
Cell Type: B cells, Dendritic cells, Endothelial cells, Fibroblasts, Granulocytes, Hematopoietic stem and progenitors, Macrophages, Monocytes, T cells
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Holness CL and Simmons DL. 1993. Blood 81:1607. 2. Gottfried E, et al. 2008. Scand. J. Immunol. 67:453. 3. Hameed A, et al. 1994. Hum. Pathol. 25:872.
Gene ID: 968
UniProt: View information about CD68 on UniProt.org
Clone: Y1/82A
Regulatory Status: RUO
Workshop: VI MR23
Other Names: Macrosialin
Isotype: Mouse IgG2b, κ