Biolegend, 333912, PerCP/Cyanine5.5 anti-human CD30 Antibody, 100tests

CD30, also known as Ki-1 antigen, lymphoid activation antigen CD30, and tumor necrosis factor receptor superfamily member 8 is a type I transmembrane receptor that contains four TNF receptor domains with an approximate molecular weight of 64 kD. CD30 is highly expressed on Hodgkins and Reed-Sternberg cells as well as activated, but not resting, T and B cells. CD30 has been shown to interact with a number of proteins including TRAF1, TRAF2, TRAF3, TRAF5, NPM-ALK, TRAF-interacting protein, and CD30 ligand (CD153). Signaling through CD30 is thought to limit the proliferative potential of autoreactive CD8 effector T cells and protect against autoimmunity.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Recombinant human CD30 boosted with THP-1 cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported application: in combination with IL-2 and PMA to induce T cell clone proliferation.
Application References(PubMed link indicates BioLegend citation): Leca G, et al. 1994. Cell. Immunol. 156:230.
RRID: AB_2566269 (BioLegend Cat. No. 333911) AB_2566270 (BioLegend Cat. No. 333912)
Structure: Type I transmembrane receptor and member of the TNF receptor superfamily that contains 4 TNF receptor domains; predicted molecular weight approximately 64 kD
Distribution: Highly expressed on Hodgkins and Reed-Sternberg cells and activated, but not resting, T cells and B cells.
Function: Signaling through CD30 is thought to limit the proliferative potential of autoreactive CD8 effector T cells and protect against autoimmunity.
Interaction: TRAF1, TRAF2, TRAF3, TRAF5, NPM-ALK, TRAF-interacting protein, CD30 ligand (CD153)
Ligand/Receptor: CD30 Ligand (CD153)
Cell Type: B cells, Embryonic Stem Cells, Tregs
Biology Area: Immunology, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Durkop H, et al. 1992. Cell 68:421. 2. Aizawa S, et al. 1997. J. Biol. Chem. 272:2042. 3. Stein H, et al. 1982. Int. J. Cancer 30:445.
Gene ID: 943
UniProt: View information about CD30 on UniProt.org
Clone: BY88
Regulatory Status: RUO
Workshop: V BP173
Other Names: Ber-H2, Ki-1, TNFRSF8
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.