Biolegend, 334103, FITC anti-human CD71 Antibody, 25tests

CD71 is a 95 kD type II homodimeric transmembrane glycoprotein also known as T9 and transferrin receptor. It is expressed on proliferating cells, reticulocytes, and erythroid precursors. CD71 plays a role in the control of cellular proliferation by facilitating the uptake of iron via ferrotransferrin binding and the recycling of apotransferrin to the cell surface.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: NALM-6 pre-B cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations: Ravichandran M, et al. 2022. Cancer Discov. 12:2198. PubMed Liu RX, et al. 2023. iScience. 26:106140. PubMed Rosato F, et al. 2022. J Transl Med. 20:578. PubMed Leick MB, et al. 2022. Cancer Cell. 40:494. PubMed Quezada-Vidal J, et al. 2020. Environ Mol Mutagen. 61:922. PubMed Park Y, et al. 2012. Biochem Biophys Res Commun. 423:19. PubMed Leimkühler NB, et al. 2020. Cell Stem Cell. 28:637. PubMed Mori Y, et al. 2015. Proc Natl Acad Sci U S A. 112: 9638-9643. PubMed Turner JS, et al. 2021. Nature. 596:109. PubMed Lin JR et al. 2018. eLife. 7 pii: e31657. PubMed Cho A, et al. 2019. Cell Rep. 28:909. PubMed Di Genua C, et al. 2020. Cancer Cell. 37(5):690-704.e8.. PubMed Amanat F, et al. 2021. Cell. 184:3936. PubMed , et al. 2021. Eur J Immunol. 51:2708. PubMed Minuesa G, et al. 2019. Nat Commun. 10:2691. PubMed Khoo NKH, et al. 2022. Med (N Y). 3:104. PubMed Madsen A, et al. 2020. Immunity. 53:852. PubMed Turner JS, et al. 2020. Nature. 586:127. PubMed Muller YD, et al. 2021. Front Immunol. 12:639818. PubMed Gleitz HFE, et al. 2021. STAR Protocols. 2(2):100538. PubMed Kim JS, et al. 2019. Bioconjug Chem. 30:1220. PubMed Yang L, et al. 2020. Nat Commun. 11:2560. PubMed Yoshinaga M, et al. 2022. Nat Commun. 13:6435. PubMed
RRID: AB_1236432 (BioLegend Cat. No. 334103) AB_2201482 (BioLegend Cat. No. 334104)
Structure: Transferrin receptor family, type II glycoprotein, 95 kD
Distribution: Proliferating cells, reticulocytes, erythroid precursors
Function: Mediates uptake of iron
Ligand/Receptor: Transferrin
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Hentze M, et al. 1996. P. Natl. Acad. Sci. USA 93:8175. 2. Trowbridge I, et al. 1993. Annu. Rev. Cell Biol. 9:129.
Gene ID: 7037
UniProt: View information about CD71 on UniProt.org
Clone: CY1G4
Regulatory Status: RUO
Workshop: A015
Other Names: T9, Transferrin receptor
Isotype: Mouse IgG2a, κ