Biolegend, 334106, PE anti-human CD71 Antibody, 100tests

CD71 is a 95 kD type II homodimeric transmembrane glycoprotein also known as T9 and transferrin receptor. It is expressed on proliferating cells, reticulocytes, and erythroid precursors. CD71 plays a role in the control of cellular proliferation by facilitating the uptake of iron via ferrotransferrin binding and the recycling of apotransferrin to the cell surface.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: NALM-6 pre-B cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations: Perik-Zavodskii R, et al. 2022. Genes (Basel). 13: . PubMed Segal JM, et al. 2019. Nat Commun. 10:3350. PubMed Angiari S, et al. 2020. Cell Metab. 31:391. PubMed Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed Pérez-Quintero L, et al. 2014. J Exp Med. 211:727. PubMed Turner JS, et al. 2021. Nature. 596:109. PubMed Himadewi P, et al. 2021. Elife. 10:. PubMed Liu S, et al. 2021. Mol Ther. 29:1918. PubMed Morrison TA, et al. 2018. Blood Cells Mol Dis. 69:1. PubMed Davis CW et al. 2019. Cell. 177(6):1566-1582 . PubMed Kanemasa H, et al. 2021. Sci Rep. 11:14396. PubMed Ochi K, et al. 2014. Stem Cells Trans Med. 3:792. PubMed Turner JS, et al. 2020. Nature. 586:127. PubMed Yang W, et al. 2015. PLoS One. 10: 0134995. PubMed Lai Y, et al. 2022. Clin Transl Med. 12:e999. PubMed Mndez-Mancilla A, et al. 2021. Cell Chemical Biology. 29:321. PubMed Hollevoet K, et al. 2014. Mol Cancer Ther. 13:2040. PubMed Hollevoet K, et al. 2015. PLoS One. 10:122462. PubMed Asada S, et al. 2018. Nat Commun. 9:2733. PubMed Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed Turner JS, et al. 2021. Nature. 595:421. PubMed Hill DL, et al. 2019. J Exp Med. 216:1857. PubMed Perik-Zavodskii R, et al. 2022. Genes (Basel). 13:. PubMed
RRID: AB_2271603 (BioLegend Cat. No. 334105) AB_2201481 (BioLegend Cat. No. 334106)
Structure: Transferrin receptor family, type II glycoprotein, 95 kD
Distribution: Proliferating cells, reticulocytes, erythroid precursors
Function: Mediates uptake of iron
Ligand/Receptor: Transferrin
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Hentze M, et al. 1996. P. Natl. Acad. Sci. USA 93:8175. 2. Trowbridge I, et al. 1993. Annu. Rev. Cell Biol. 9:129.
Gene ID: 7037
UniProt: View information about CD71 on UniProt.org
Clone: CY1G4
Regulatory Status: RUO
Workshop: A015
Other Names: T9, Transferrin receptor
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.