Biolegend, 334111, PE/Cyanine7 anti-human CD71 Antibody, 25tests

CD71 is a 95 kD type II homodimeric transmembrane glycoprotein also known as T9 and transferrin receptor. It is expressed on proliferating cells, reticulocytes, and erythroid precursors. CD71 plays a role in the control of cellular proliferation by facilitating the uptake of iron via ferrotransferrin binding and the recycling of apotransferrin to the cell surface.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: NALM-6 pre-B cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations: Leng K, et al. 2022. Nat Neurosci. 25:1528. PubMed Anderson W, et al. 2023. Elife. 12:. PubMed Qin K, et al. 2022. Nat Genet. 54:874. PubMed Koutsakos M, et al. 2022. Immunity. 55:1316. PubMed Mastelic-Gavillet B, et al. 2019. J Immunother Cancer. 7:257. PubMed Semesta KM, et al. 2020. PLoS Genet. 16:e1009103. PubMed Turner JS, et al. 2021. Nature. 596:109. PubMed Caielli S, et al. 2021. Cell. 184(17):4464-4479.e19. PubMed He X, et al. 2021. Emerg Microbes Infect. 10:1555. PubMed Lan X, et al. 2020. Molecular Cell. 81(2):239-254.e8. PubMed Dräger NM, et al. 2022. Nat Neurosci. 25:1149. PubMed Auladell M, et al. 2019. Clin Transl Immunology. 8:e01090. PubMed Martin RM et al. 2019. Cell Stem Cell. 24(5):821-828 . PubMed Sun D, et al. 2021. Elife. 10:. PubMed Sokal A, et al. 2021. Cell. 184(5):1201-1213.e14. PubMed Mudd PA, et al. 2022. Cell. 185:603. PubMed Turner JS, et al. 2021. Nature. 595:421. PubMed Sokal A, et al. 2021. Immunity. S1074-7613:00396. PubMed
RRID: AB_2563118 (BioLegend Cat. No. 334111) AB_2563119 (BioLegend Cat. No. 334112)
Structure: Transferrin receptor family, type II glycoprotein, 95 kD
Distribution: Proliferating cells, reticulocytes, erythroid precursors
Function: Mediates uptake of iron
Ligand/Receptor: Transferrin
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Hentze M, et al. 1996. P. Natl. Acad. Sci. USA 93:8175. 2. Trowbridge I, et al. 1993. Annu. Rev. Cell Biol. 9:129.
Gene ID: 7037
UniProt: View information about CD71 on UniProt.org
Clone: CY1G4
Regulatory Status: RUO
Workshop: A015
Other Names: T9, Transferrin receptor
Isotype: Mouse IgG2a, κ