Biolegend, 334301, Purified anti-human CD40 Antibody, 25μg

CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The 5C3 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially blocking CD40 binding to CD40L, and B cells rescue from apoptosis.
25μg
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: Baboon, Chimpanzee, Squirrel Monkey
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested Costim - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (for the relevant formats) include: costimulation of B cell proliferation1, partial inhibition of CD40 binding to CD40L3, and B cell rescue from apoptosis1. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.
Application References(PubMed link indicates BioLegend citation): Schlossman SF, et al. 1995. ed. Leukocyte Typing V:White Cell Differentiation Antigens. New York:Oxford University Press. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Pound JD, et al. 1999. Int. Immunol. 11:11. Shey MS, et al. 2014. J Immunol. 192:4833. PubMed Sondergaard JN, et al. 2014. Mol Immunol. 59:180. PubMed Saiki O, et al. 1995. J Clin. Invest. 2: 510-4 (Costim)
Product Citations: Silvin A, et al. 2020. Cell. 182:1401. PubMed Tanner MR, et al. 2019. Arthritis Res Ther. 21:6. PubMed McCarthy EE, et al. 2022. Cell Rep. 39:110815. PubMed Trotta A, et al. 2018. Front Immunol. 1.069444444. PubMed Mask E, et al. 2022. Front Cell Infect Microbiol. 12:880860. PubMed Heesters BA, et al. 2021. J Exp Med. 218:. PubMed Bruno TC, et al. 2017. Cancer Immunol Res. 0.831944444. PubMed Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed Warncke M, et al. 2012. J Immunol. 188:4405. PubMed Lee-Chang C, et al. 2021. J Exp Med. 218:. PubMed Zimmerman MG, et al. 2018. Cell Host Microbe. 24:731. PubMed
RRID: AB_1236384 (BioLegend Cat. No. 334302)
Structure: TNFR superfamily, type I glycoprotein, 48 kD
Distribution: B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts
Function: B cell differentiation, costimulation, isotype class-switching, rescues B cells from apoptosis
Ligand/Receptor: CD154 (CD40 ligand)
Cell Type: B cells, Dendritic cells, Endothelial cells, Fibroblasts, Macrophages
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules
Antigen References: 1. Banchereau J, et al. 1994. Annu. Rev. Immunol. 12:881. 2. Foy T, et al. 1996. Annu. Rev. Immunol. 14:591.
Gene ID: 958
UniProt: View information about CD40 on UniProt.org
Clone: 5C3
Regulatory Status: RUO
Workshop: V CD40.4
Other Names: BP50, TNFRSF5
Isotype: Mouse IgG1, κ