Biolegend, 334404, PE anti-human CD245 (p220/240) Antibody, 100tests

CD245 is a 220-250 kD transmembrane glycoprotein, also known as p220/240, DY12 or DY35. It is expressed on peripheral blood lymphocytes, monocytes, granulocytes, and platelets. The expression level of CD245 on lymphocytes can be upregulated after 3-7 days stimulation with PHA. It has been reported that CD245 is involved in signal transduction and costimulation of T and NK cells.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_1186064 (BioLegend Cat. No. 334403)
Distribution: Blood lymphocytes, monocytes and granulocytes
Function: Signal transduction and costimulation
Cell Type: Granulocytes, Lymphocytes, Monocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. H. Zola et al. Eds. 2007. Leukocyte and Stromal Cell Molecule:The CD Markers. WILEY-LISS:A John Wiley & Sons Inc, Publication
Gene ID: 4863
UniProt: View information about CD245 on UniProt.org
Clone: DY12
Regulatory Status: RUO
Other Names: P220/240, DY12, DY35
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.