Biolegend, 334802, Purified anti-human CD300c Antibody, 100μg

CD300c, also known as CMRF-35-like molecule 6 (CLM-6) and leukocyte immunoglobulin-like receptor (LIR) is a 25 kD protein that belongs to the myeloid-associated immunoglobulin-like receptor (MAIR) family of genes. This transmembrane protein is expressed on certain B cells, T cells, NK cells, granulocytes, macrophages, monocytes, and mast cells. CD300C promotes secretion of proinflammatory cytokines and is an activating immune receptor associated ITAM-bearing adapter DAP12 and FcRγ chain on macrophages.
100μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Simhadri VR, et al. 2013. J. Innate Immun. 4:389.
RRID: AB_2303773 (BioLegend Cat. No. 334802)
Structure: Ig superfamily, 40 kD
Distribution: Monocytes/macrophages, dendritic cells
Function: Regulate myeloid cell activation and promote secretion of cytokines and chemokines
Interaction: DAP12, FcRγ
Cell Type: Dendritic cells, Macrophages, Monocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Borrego F. 2013. Blood 11:1951. 2. Ju X, et al. 2009. Immunobiology 730-736. 3. Simhadri, et al. 2011. BMC Immunology 12:62.
Gene ID: 10871
UniProt: View information about CD300c on UniProt.org
Clone: TX45
Regulatory Status: RUO
Other Names: LIR, CLM-6, CMRF35, IGSF16, CMRF-35, CMRF35A, CMRF-35A, CMRF35A1, CMRF35-A1
Isotype: Mouse IgG1, κ