Biolegend, 334803, PE anti-human CD300c Antibody, 25tests

CD300c, also known as CMRF-35-like molecule 6 (CLM-6) and leukocyte immunoglobulin-like receptor (LIR) is a 25 kD protein that belongs to the myeloid-associated immunoglobulin-like receptor (MAIR) family of genes. This transmembrane protein is expressed on certain B cells, T cells, NK cells, granulocytes, macrophages, monocytes, and mast cells. CD300C promotes secretion of proinflammatory cytokines and is an activating immune receptor associated ITAM-bearing adapter DAP12 and FcRγ chain on macrophages.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Simhadri VR, et al. 2013. J. Innate Immun. 4:389.
Product Citations: Cheng WC, et al. 2016. Eur J Immunol. 2211:46. PubMed
RRID: AB_2208159 (BioLegend Cat. No. 334804)
Structure: Ig superfamily, 40 kD
Distribution: Monocytes/macrophages, dendritic cells
Function: Regulate myeloid cell activation and promote secretion of cytokines and chemokines
Interaction: DAP12, FcRγ
Cell Type: Dendritic cells, Macrophages, Monocytes
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Borrego F. 2013. Blood 11:1951. 2. Ju X, et al. 2009. Immunobiology 730-736. 3. Simhadri, et al. 2011. BMC Immunology 12:62.
Gene ID: 10871
UniProt: View information about CD300c on UniProt.org
Clone: TX45
Regulatory Status: RUO
Other Names: LIR, CLM-6, CMRF35, IGSF16, CMRF-35, CMRF35A, CMRF-35A, CMRF35A1, CMRF35-A1
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.