Biolegend, 335105, APC anti-human CD158i (KIR2DS4) Antibody, 25tests

CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins that are expressed on specific subsets of NK and T cells. KIRs are characterized as having either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Some KIRs with long cytoplasmic domains contain ITIMs and possess inhibitory functions, while others with short cytoplasmic region lack ITIMs and have activating functions. Fifteen polymorphic KIR genes have been identified in humans, contributing to the extensive diversity of KIR phenotypes observed in individuals. CD158i is an activating receptor with two Ig-like domains and a short cytoplasmic region. KIR2DS4 is reported to bind to certain HLA-C molecules and HLA-A*11.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KIR2DS4-transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Based on in-house testing on different KIR-expressing transfectants, clone S22012A does not interact with other KIR protein family members.
RRID: AB_3698986 (BioLegend Cat. No. 335105) AB_3698986 (BioLegend Cat. No. 335106)
Structure: Two Ig-like extracellular domain glycoprotein
Distribution: Subset of NK cells, subset of T Cells
Function: NK cell regulation
Ligand/Receptor: HLA-C, HLA-A*11
Cell Type: Lymphocytes, NK cells, T cells
Biology Area: Cell Biology, Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: Bottino C, et al. 1996. Eur J immunol. 26:1816-24. Graef T, et al. 2009. J Exp Med. 206:2557-72. Campbell KS, et al. 1998. Eur J Immunol. 28:599-609.
Gene ID: 3809
UniProt: View information about CD158i on UniProt.org
Clone: S22012A
Regulatory Status: RUO
Other Names: NKAT8, Killer Cell Immunoglobulin-Like Receptor 2DS4
Isotype: Mouse IgG1, κ