Biolegend, 335306, Purified anti-human CD26 Antibody, 100μg

CD26, also known as ADA-binding protein and dipeptidyl peptidase IV (DPPIV), is a 110 kD type II membrane protein. It is a member of the peptidase and ectoenzyme family. CD26 is expressed on the membrane of mature thymocytes, T lymphocytes (upregulated upon activation), B cells, NK cells, and macrophages. CD26 cleaves off N-terminal X-Pro and X-Ala dipeptides from polypeptides. It plays an integral role as a costimulatory molecule in T cell activation. CD26 may interact with extracellular matrix proteins such as fibronectin or collagen, CD45, and ADA.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested Direct ELISA – Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. Each lot of this antibody is verified by Direct ELISA. The optimal concentration of this reagent for the specific application and the experiment design should be established by the end-user.
Application Notes: Test clone 5-109 and reference clone BA5b completely cross-block the staining on target cells.
Additional Product Notes: This product is intended for sandwich-based immunoassay development on various assay platforms requiring antibody pairs. This antibody (clone 5-109) could function as a capture antibody when paired with biotin anti-human CD26 (DDP4) (Cat No. 628153) (clone 1-10) as the detection antibody and using recombinant Human DDP4 (carrier-free) protein (Cat. Nos. 764102, 764104, and 764106) as the assay standard.
RRID: AB_3662413 (BioLegend Cat. No. 335306)
Structure: Peptidases and ectoenzyme families, type II glycoprotein, 110 kD
Distribution: Thymocyte subset, memory T cells, B cells, NK cells, epithelial cells, macrophages
Function: Dipeptidyl peptidase, T cell costimulation, HIV entry
Ligand/Receptor: Adenosine-deaminase, collagen
Cell Type: B cells, Epithelial cells, Macrophages, NK cells, T cells, Thymocytes
Biology Area: Costimulatory Molecules, Immunology
Molecular Family: CD Molecules
Antigen References: Kameoka J, et al. 1993. Science. 261:466-9. Dang NH, et al. 1990. J Exp Med. 172:649-52.
Gene ID: 1803
UniProt: View information about CD26 on UniProt.org
Clone: 5-109
Regulatory Status: RUO
Other Names: ADA-binding protein, DPP IV ectoenzyme, EC 3.4.14.5, DPP4
Isotype: Mouse IgG1, κ