Biolegend, 335511, PE anti-human CD98 Recombinant Antibody, 25tests

CD98, also known as 4F2 and FRP-1, is a 125 kD disulfide-linked heterodimer protein. It is broadly expressed on activated and transformed cells (hematopoietic and non-hematopoietic), and at low levels on quiescent cells. CD98 has been characterized as a potential amino acid transporter (neutral and dibasic amino acids) that is involved in lymphocyte activation and integrin signaling. CD98 is composed of a light chain (45 kD) and a heavy chain (80 kD). Studies with knock-out mice indicate that the CD98 heavy chain is involved in integrin-dependent cell spreading and protection against anchorage deprivation-induced apoptosis.
25tests
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_3675055 (BioLegend Cat. No. 335511) AB_3675055 (BioLegend Cat. No. 335512)
Structure: Disulfide-linked heterodimer, 80 kD and 45 kD reduced, 125 kD unreduced
Distribution: Broadly expressed on activated and transformed cells, not hematopoietic cell-specific. Lower levels on resting cells.
Function: Potential amino acid transporter (neutral and dibasic amino acids) also involved in the activation of lymphocytes and integrin signaling. May be a target antigen for NK cells.
Interaction: Actin, β1 integrins (α2β1, α3β1, α5β1, α6β1; minimally with α4β1)
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: Leukocyte Typing VI. Kishimoto T, et al. (Eds.) Garland Publishing Inc. (1997) Bron C, et al. 1986. J. Immunol. 137:397. Lumadue JA, et al. 1987. Proc. Natl. Acad. Sci. 84:9204. Mastroberardino L, et al. 1998. Nature 395:288.
Gene ID: 6520
UniProt: View information about CD98 on UniProt.org
Clone: 2B6/CD98.Rec
Regulatory Status: RUO
Other Names: 4F2, FRP-1, RL-388
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.