Biolegend, 335613, PE anti-human EGFRvIII Recombinant Antibody, 25tests

Epidermal growth factor receptor variant III (EGFRvIII) arises from an in-frame deletion of exons 2 to 7 in the extracellular region of EGFR, resulting in a truncated receptor that is constitutively active. This mutation is cancer-specific and is present in approximately 50% of glioblastoma tumors.
25tests
Verified Reactivity: Human
Antibody Type: Recombinant
Host Species: Mouse
Immunogen: Peptide LEEKKGNYVVTDHC conjugated to KLH
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID: AB_3675057 (BioLegend Cat. No. 335613) AB_3675057 (BioLegend Cat. No. 335614)
Structure: Deletion of exons 2-7 of the EGFR gene. Molecular mass of approximately 145 kD.
Distribution: Present in approximately 50% of glioblastoma tumors
Function: Pro-tumorigenic (promotes tumor growth, survival, invasion, stemness, metabolism, and angiogenesis).
Biology Area: Cancer Biomarkers
Antigen References: Hui G, et al. 2013. FEBS Journal. 280:21.
Gene ID: 1956
UniProt: View information about EGFRvIII on UniProt.org
Clone: DH8.3.Rec
Regulatory Status: RUO
Other Names: EGFRv3
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.