Biolegend, 335911, PE/Cyanine7 anti-human HLA-G Antibody, 25tests

HLA-G is a nonclassical MHC class I (MHC-Ib) molecule structurally related to MHC class Ia (HLA-A,B, C). There are seven isoforms of this molecule, including membrane bound HLA-G1, -G2, -G3 and -G4 and soluble HLA-G5, -G6, and -G7. HLA-G is primarily expressed on trophoblast cells. Its expression is also found on thymic epithelial cells, cytokine-activated moncytes, macrophages/dendritic cells during viral infection, and in various tumors. HLA-G exerts its inhibitory functions to regulate immune tolerance via interaction with inhibitory receptors ILT2(CD85j), ILT4(CD85d), and KIR2DL4(CD158d), which is expressed on NK cells, T cells, monocytes, dendritic cells, and B cells. HLA-G is also able to bind CD8α/α, which may mediate positive and/or negative selection in thymus.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: HLA-G transfected cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: 87G mAb reacts with isoforms of HLA-G1 and -G5. Additional reported applications (for the relevant formats) include: restoring HLA-G mediated suppression of allo-T cell proliferation and immunohistochemical staining of frozen tissue sections. Clone 87G is reported not to bind HLA-G in aldehyde fixed samples.4
Application References(PubMed link indicates BioLegend citation): Odum N, et al. 1991. Eur. J. Immunol. 21:2121. Lila N, et al. 2001. P. Natl. Acad. Sci. USA 98:12150. Lila N, et al. 2002. Circulation. 105:1949. Blaschitz A, et al. 2000. Hum. Immunol. 61:1074. Balsamo M, et al. 2012. Eur J. Immunol. 42:1833. PubMed
Product Citations: Manukyan G, et al. 2020. Auto Immun Highlights. 11:5. PubMed
RRID: AB_2561343 (BioLegend Cat. No. 335911) AB_2561344 (BioLegend Cat. No. 335912)
Structure: Ig
Distribution: Trophoblast cells, thymic epithelial cells, cytokine-activated moncytes, macrophages/dendritic cells during viral infection, various tumors
Function: Inhibit NK and T cell-mediated cytolysis and T cell proliferation, induce apoptosis of activated CD8+ T cells, induce Th2 cytokine production
Ligand/Receptor: ILT2(CD85j), ILT4(CD85d), KIR2DL4(CD158d), CD8α/α
Cell Type: Dendritic cells, Epithelial cells, Macrophages, Monocytes
Biology Area: Immunology
Molecular Family: MHC Antigens
Antigen References: 1. Hunt JS, et al. 2005. FASEB J. 19:681. 2. Carosella ED, et al. 2008. Blood 111:4862.
Gene ID: 3135
UniProt: View information about HLA-G on UniProt.org
Clone: 87G
Regulatory Status: RUO
Other Names: Human Leukocyte Antigen-G
Isotype: Mouse IgG2a, κ