Biolegend, 336307, PE anti-human CD97 Antibody, 25tests

CD97 is a seven-span transmembrane protein belonging to the secretin receptor superfamily, the G-protein-coupled receptor superfamily, and the EGF-TM7 family with molecular weights of 74 kD, 80 kD, and 86 kD. It is expressed on monocytes/macrophages, granulocytes, dendritic cells, and smooth muscle cells.The expression of CD97 on resting T- and B-lymphocytes is at low level, but is rapidly upregulated upon activation. CD97 binds to CD55 (decay-accelerating factor), chondroitin sulfate, integrin a5b1, and a3b1. CD97 has been shown to mediate cell adhesion and co-stimulation of T cell proliferation.
25tests
Verified Reactivity: Human
Reported Reactivity: Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: KG1a (human myeloid cell line)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone VIM3b bindswithin the first EGF domain of CD97.4 Additional reported applications include: western blot, immunoprecipitation.
Application References(PubMed link indicates BioLegend citation): Hamann J, et al. 1995. J. Immunol. 155:1942. Lawrence DW, et al. 2003. J. Exp. Med. 198:999. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Wobus M, et al. 2004. Int. J. Cancer 112:815.
Product Citations: Nakajima H, et al. 2007. J Neurochem. 2428:103. PubMed de Boer B et al. 2018. Cancer cell. 34(4):674-689 . PubMed Houtsma R, et al. 2021. STAR Protoc. 2:100864. PubMed
RRID: AB_1227612 (BioLegend Cat. No. 336307) AB_2076182 (BioLegend Cat. No. 336308)
Structure: Seven-span transmembrane protein belonging to the secretin receptor superfamily, the G-protein-coupled receptor superfamily, and the EGF-TM7 family, 74 kD, 80 kD, 86 kD
Distribution: Monocytes/macrophages, granulocytes, dendritic cells, low levels on T cells and B-lymphocytes and upregulated upon activation
Function: Co-stimulation, adhesion
Ligand/Receptor: CD55, chondroitin sulfate, integrin α5β1, integrin α3β1
Cell Type: Dendritic cells, Granulocytes, Macrophages, Monocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Capasso M, et al. 2006. J. Immunol. 177:1070 2. Wang T, et al. 2005. Blood 105:2836 3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. A John Wiley & Sons Inc, Publication
Gene ID: 976
UniProt: View information about CD97 on UniProt.org
Clone: VIM3b
Regulatory Status: RUO
Workshop: V BP030
Other Names: BL-KDD/F12
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.