Biolegend, 336413, Purified anti-human CD61 (Maxpar® Ready) Antibody, 100μg

CD61, also known as integrin β3 and glycoprotein IIIa (gpIIIa), is a 90 kD type I integral transmembrane glycoprotein. It is a member of the integrin family, associating with platelet gpIIb (CD41) to form CD41/CD61 complex and with integrin αV (CD51) to form αV/β3 (CD51/CD61) integrin. CD41/CD61 is expressed on platelets and megakaryocytes, and plays a role in platelet activation and aggregation through interaction with fibrinogen, fibronectin, vWF, and other RGD-containing adhesion molecules. CD51/CD61 is expressed on platelets, osteoclasts, fibroblasts, macrophages, and some tumor cells involved in tumor metastasis, and in adenovirus infection through binding to RGD motif in extracellular matrix proteins.
100μg
Verified Reactivity: Human, Cynomolgus, Rhesus
Reported Reactivity: African Green, Baboon
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 1.0 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested CyTOF® - Verified
Recommended Usage: This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting and immunohistochemical staining of frozen tissue sections.
Additional Product Notes: Maxpar® is a registered trademark of Standard BioTools Inc.
Application References(PubMed link indicates BioLegend citation): Davies J, et al. 1989. J. Cell Biol. 109:1817. Roberts M, et al. 2004. Mol. Cell. Biol. 24:1505. Ciarlet M, et al. 2002. J. Virol. 76:1109.
RRID: AB_2562833 (BioLegend Cat. No. 336413)
Structure: Type I integral glycoprotein, integrin family, associates with CD41 (gpIIb) forming CD41/CD61 complex or with CD51 (integrin αV) forming CD51/CD61 complex, 90 kD
Distribution: CD41/CD61 complex is expressed on platelets and megakaryocytes; CD51/CD61 complex is expressed on platelets, osteoclasts, fibroblasts, macrophages, and some tumor cells
Function: Adhesion, platelet activation and aggregation
Ligand/Receptor: Fibronectin, vitronectin, vWF
Cell Type: Fibroblasts, Megakaryocytes, Osteoclasts, Platelets
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers.
Gene ID: 3690
UniProt: View information about CD61 on UniProt.org
Clone: VI-PL2
Regulatory Status: RUO
Other Names: Integrin β3, gpIIIa
Isotype: Mouse IgG1, κ
Q: Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?
A: We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm®. Please contact Fluidigm® directly for additional data and further details.

http://techsupport.fluidigm.com/
Q: Can I use Maxpar® Ready format clones for flow cytometry staining?
A: We have not tested the Maxpar® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.
Q: I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.
A: We only supply the antibody and not test that in house. Please contact Fluidigm® directly for troubleshooting advice: http://techsupport.fluidigm.com/
Q: Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?
A: The Maxpar® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.