Biolegend, 336716, PerCP/Cyanine5.5 anti-human CD48 Antibody, 100tests

CD48 is a 40-47 kD GPI-anchored membrane protein, also known as Blast-1 and HuLy-m3. It is a member of the CD2 family that contains 2 IgSF domains and is widely expressed on both resting and activated hematopoietic cells with the exception of granulocytes, platelets, and erythrocytes. CD48 binds to CD2 at a considerably (>100-fold) lower affinity than CD58. It is thought to contribute to T cell activation. The cytoplasmic tail of CD48 has been shown to bind to the kinases Lck and Fyn.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation.
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. 1997. Leucocyte Typing VI:White Cell Differentiation Antigens. Garland Publishing Inc. Wang R, et al. 2012. J. Leukoc Biol. 91:299. PubMed
RRID: AB_2810518 (BioLegend Cat. No. 336715) AB_2810519 (BioLegend Cat. No. 336716)
Structure: CD2 family, contains 2 IgSF domains, GPI-membrane anchored, 40-47 kD
Distribution: Widely expressed on hematopoietic cells except granulocytes, platelets, and erythrocytes. Increased on T and B cells after activation
Function: Contributes to T cell antigen recognition
Ligand/Receptor: CD2
Cell Targets: Lck, Fyn interact with cytoplasmic domain
Cell Type: B cells
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: Fisher RC and Thorley-Lawson DA. 1991. Mol. Cell. Biol. 11:1614. Korinek V, et al. 1991. Immunogenetics 33:108. Leukocyte Typing IV. Knapp W, et al. (Eds) Oxford University Press (1989) Leukocyte Typing V. Schlossman S, et al. (Eds) Oxford University Press (1995)
Gene ID: 962
UniProt: View information about CD48 on UniProt.org
Clone: BJ40
Regulatory Status: RUO
Other Names: Blast-1, SLAMF2, HuLy-m3
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.