Biolegend, 336902, Purified anti-human CD87 Antibody, 100μg

CD87, known as urokinase plasminogen activator-receptor (uPAR), is a 36-68 kD single-chain glycophosphotidylinositol (GPI)-anchored glycoprotein. The extracellular region of CD87 consists of three homologous domains, D1, D2, and D3. Membrane-bound and soluble (D2D3) forms of uPAR have been reported. The membrane-bound CD87 is expressed on granulocytes, monocytes/macrophages, dendritic cells, fibroblasts, endothelial cells and keratinocytes. CD87 plays a role in cell adhesion and chemotaxis through binding to β1, β2 and β3 integrins. CD87-bound uPA converts plasminogen to plasmin.
100μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: THP-1 (human myeloid cell line)
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications include: VIM5 mAb binds to uPA binding-domain
Application References(PubMed link indicates BioLegend citation): Schlossmant SF, et al. eds. 1995 Leukocyte Type V. Oxford University Press Inc, New York Gadd SJ, et al. 1990. Clin. Exp. Immunol 80:252 Sillaber C, et al. 1997. J. Biol. Chem. 272:7824
Product Citations: Dutertre CA, et al. 2020. Immunity. 51(3):573-589.e8.. PubMed
RRID: AB_2165470 (BioLegend Cat. No. 336902)
Structure: Glycophosphotidylinositol (GPI)-anchored glycoprotein, 36-68 kD
Distribution: Granulocytes, monocytes/macrophages, dendritic cells, fibroblasts, endothelial cells, keratinocytes.
Function: Convert plasminogen to plasmin, cell adhesion, chemotaxis
Ligand/Receptor: Urokinase plasminogen activator (uPA), vitronectin, β1, β2 and β3 integrins, kininogen
Cell Type: Dendritic cells, Endothelial cells, Fibroblasts, Granulocytes, Macrophages, Monocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Beaufort N, et al. 2004. J. Immunol. 172:540 2. Tarui T, et al. 2001. J. Bio. Chem. 276:3983 3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. A John Wiley & Sons Inc, Publication
Gene ID: 5329
UniProt: View information about CD87 on UniProt.org
Clone: VIM5
Regulatory Status: RUO
Workshop: VI MR13
Other Names: Urokinase plasminogen activator-receptor, uPA-R, uPAR
Isotype: Mouse IgG1, κ