Biolegend, 337209, PerCP/Cyanine5.5 anti-human CD11c Antibody, 25tests

CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin α x and CR4. CD11c non-covalently associates with integrin β 2 (CD18) and is expressed on monocytes/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. CD11c has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. * PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximun emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone Bu15 has a different binding epitope than clone 3.9. The binding of Bu15 with CD11c is divalent cation independent. Additional reported applications (for the relevant formats of this clone) include: inhibition of CD11c mediated adhesion and stimulation of chemokine production by monocytes.
Application References(PubMed link indicates BioLegend citation): Sadhu C, et al. 2008. J. Immunoass. Immunoch. 29:42. Rezzonico R, et al. 2001. Blood 97:2932. Sadhu C, et al. 2007. J. Leukoc. Biol. 81:1395. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations: Chu C, et al. 2012. J Exp Med. 209:935. PubMed Weisberg SP, et al. 2020. Cell Reports. 29(12):3916-3932.e5.. PubMed Jost M, et al. 2021. eLife. 10:00. PubMed Shey M, et al. 2014. J Immunol. 192:4833. PubMed DuMont A, et al. 2013. Proc Natl Acad Sci U S A. 110:10794. PubMed Baguma R, et al. 2017. PLoS One. 10.1371/journal.pone.0184563. PubMed
RRID: AB_1279071 (BioLegend Cat. No. 337209) AB_1279069 (BioLegend Cat. No. 337210)
Structure: Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 145-150 kD
Distribution: Myeloid, dendritic cells, NK cells, B cells and T cell subsets
Function: Adhesion, CTL killing Ligand Receptor: CD54, fibrinogen, iC3b, ICAM-1, ICAM-4 Antigen
Cell Type: B cells, Dendritic cells, Neutrophils, NK cells, T cells
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Petty H. 1996. Immunol. Today 17:209. 2. Springer T. 1994. Cell 76:301. 3. Ihanus E, et al. 2007. Blood 109:802-810.
Gene ID: 3687
UniProt: View information about CD11c on UniProt.org
Clone: Bu15
Regulatory Status: RUO
Workshop: V S143
Other Names: Integrin αx subunit, ITGAX, CR4, p150
Isotype: Mouse IgG1, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.