Biolegend, 337216, PE/Cyanine7 anti-human CD11c Antibody, 100tests

CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin α x and CR4. CD11c non-covalently associates with integrin β 2 (CD18) and is expressed on monocytes/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. CD11c has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Clone Bu15 has a different binding epitope than clone 3.9. The binding of Bu15 with CD11c is divalent cation independent. Additional reported applications (for the relevant formats of this clone) include: inhibition of CD11c mediated adhesion and stimulation of chemokine production by monocytes.
Application References(PubMed link indicates BioLegend citation): Sadhu C, et al. 2008. J. Immunoass. Immunoch. 29:42. Rezzonico R, et al. 2001. Blood 97:2932. Sadhu C, et al. 2007. J. Leukoc. Biol. 81:1395. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations: Korniotis S, et al. 2022. J Cell Sci. 135:. PubMed Brouiller F, et al. 2023. iScience. 26:106019. PubMed Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed Fournier N, et al. 2018. MAbs. 10:651. PubMed Oostindie SC, et al. 2022. Nat Biotechnol. :. PubMed Hagen SH, et al. 2020. Cell Rep. 33:108485. PubMed Sutton HJ, et al. 2021. Cell Reports. 34(6):108684. PubMed Tang-Huau TL, et al. 2018. Nat Commun. 9:2570. PubMed Bourdely P, et al. 2020. Immunity. 53(2):335-352. PubMed Leylek R, et al. 2019. Cell Rep. 29:3736. PubMed Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed Autenrieth S, et al. 2015. Clin Transl Immunology. 4:50. PubMed Hagen SH, et al. 2021. STAR Protocols. 2(3):100641. PubMed Richardson JR, et al. 2018. Front Immunol. 2.182638889. PubMed Prendergast B, et al. 2013. Brain Behav Immun. 32:104. PubMed Mackroth M, et al. 2016. PLoS Pathog. 12:e1005909. PubMed Lahaye X et al. 2018. Cell. 175(2):488-501 . PubMed Kurilin V, et al. 2022. Mol Clin Oncol. 17:155. PubMed
RRID: AB_2129791 (BioLegend Cat. No. 337215) AB_2129790 (BioLegend Cat. No. 337216)
Structure: Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 145-150 kD
Distribution: Myeloid, dendritic cells, NK cells, B cells and T cell subsets
Function: Adhesion, CTL killing Ligand Receptor: CD54, fibrinogen, iC3b, ICAM-1, ICAM-4 Antigen
Cell Type: B cells, Dendritic cells, Neutrophils, NK cells, T cells
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Petty H. 1996. Immunol. Today 17:209. 2. Springer T. 1994. Cell 76:301. 3. Ihanus E, et al. 2007. Blood 109:802-810.
Gene ID: 3687
UniProt: View information about CD11c on UniProt.org
Clone: Bu15
Regulatory Status: RUO
Workshop: V S143
Other Names: Integrin αx subunit, ITGAX, CR4, p150
Isotype: Mouse IgG1, κ