Biolegend, 337217, APC/Cyanine7 anti-human CD11c Antibody, 25tests

CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin α x and CR4. CD11c non-covalently associates with integrin β 2 (CD18) and is expressed on monocytes/macrophages, dendritic cells, granulocytes, NK cells, and subsets of T and B cells. CD11c has been reported to play a role in adhesion and CTL killing through its interactions with fibrinogen, CD54, and iC3b.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: Clone Bu15 has a different binding epitope than clone 3.9. The binding of Bu15 with CD11c is divalent cation independent. Additional reported applications (for the relevant formats of this clone) include: inhibition of CD11c mediated adhesion and stimulation of chemokine production by monocytes.
Application References(PubMed link indicates BioLegend citation): Sadhu C, et al. 2008. J. Immunoass. Immunoch. 29:42. Rezzonico R, et al. 2001. Blood 97:2932. Sadhu C, et al. 2007. J. Leukoc. Biol. 81:1395. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Product Citations: Wu X, et al. 2023. iScience. 26:106559. PubMed De Hert E, et al. 2022. Int J Mol Sci. 23:. PubMed Cavalli G, et al. 2016. Proc Natl Acad Sci U S A. 113: 1363 - 1368. PubMed Canavan M, et al. 2018. JCI Insight. 3:. PubMed Liu C, et al. 2021. Cell Res. 31:1106. PubMed Izquierdo-Useros N, et al. 2012. PLoS One. 10:e1001315. PubMed Forbester JL, et al. 2020. J Virol. 94:. PubMed Wouters K, et al. 2017. Sci Rep. 7:42665. PubMed Grandclaudon M, et al. 2019. Cell. 179:432. PubMed Kaur A, et al. 2019. Cancer Discov. 9:64. PubMed Fredriksson K, et al. 2014. J Immunol. 192:4497. PubMed Richardson JR, et al. 2018. Front Immunol. 2.182638889. PubMed Verboven K, et al. 2018. Sci Rep. 8:4677. PubMed Zhang X, et al. 2021. Nat Commun. 12:4536. PubMed Céspedes PF, et al. 2022. Nat Commun. 13:3460. PubMed Jardine L, et al. 2019. Nat Commun. 10:1999. PubMed Koh WH, et al. 2020. STAR Protoc. 1:100203. PubMed Ceglia V, et al. 2021. J Immunol. 207:2060. PubMed Milne P, et al. 2015. Blood. 125:470. PubMed Zimmermann M, et al. 2019. Front Immunol. 10:829. PubMed Cytlak U, et al. 2020. Immunity. 53(2):353-370. PubMed Ceglia V, et al. 2022. Front Immunol. 12:786144. PubMed
RRID: AB_10661724 (BioLegend Cat. No. 337217) AB_10662746 (BioLegend Cat. No. 337218)
Structure: Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 145-150 kD
Distribution: Myeloid, dendritic cells, NK cells, B cells and T cell subsets
Function: Adhesion, CTL killing Ligand Receptor: CD54, fibrinogen, iC3b, ICAM-1, ICAM-4 Antigen
Cell Type: B cells, Dendritic cells, Neutrophils, NK cells, T cells
Biology Area: Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Petty H. 1996. Immunol. Today 17:209. 2. Springer T. 1994. Cell 76:301. 3. Ihanus E, et al. 2007. Blood 109:802-810.
Gene ID: 3687
UniProt: View information about CD11c on UniProt.org
Clone: Bu15
Regulatory Status: RUO
Workshop: V S143
Other Names: Integrin αx subunit, ITGAX, CR4, p150
Isotype: Mouse IgG1, κ