Biolegend, 337424, Ultra-LEAF™ Purified anti-human CD112 (Nectin-2) Antibody, 1mg

CD112, known as poliovirus receptor related 2 protein (PRR2), Nectin-2, or Hve B, is a type I transmembrane glycoprotein, a member of the Ig gene superfamily. It is primarily found on myelomonocytic cells, megakaryocytes, dendritic cells, mast cells, CD34 positive stem cells, endothelial cells, epithelial cells, and neuronal cells. CD112 functions as a receptor for α-herpes viruses HSV-1 and pseudorabies virus (PRV). CD112 is an adhesion molecule involved in the formation of cell junction and regulation of NK- and T cell-mediated cytotoxicity through interaction with CD226 (DNAM-1) and other nectin family molecules.
1mg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality testedBlock - Verified
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. The antibody also blocks the binding between recombinant human CD112/Nectin-2 and recombinant human CD112R (Cat. No. 772106). The ND50 range is 0.8 - 5.0 μg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications include: partially block CD226-Fc binding to CD112 transfectant cells and partially inhibit the cytotoxicity mediated by NK and T cells.
Application References(PubMed link indicates BioLegend citation): Tahara-Hanaoka S, et al.. 2004. Int. Immunol. 16:533 Lim O, et al. 2013. PLoS One. 8:e53611. PubMed Gillory LA, 2013. PLoS One. 8:77753. PubMed
RRID: AB_2819965 (BioLegend Cat. No. 337423) AB_2819966 (BioLegend Cat. No. 337424)
Structure: Type I transmembrane glycoprotein, Ig superfamily
Distribution: Myelomonocytic cells, megakaryocytes, dendritic cells, mast cells, CD34-positive stem cells, endothelial cells, epithelial cells, and neuronal cells.
Function: Adhesion, receptor for some Herpes Viruses
Ligand/Receptor: CD226, CD155, Nectin-1, -3, and -4, Herpes Viruses
Cell Type: Dendritic cells, Endothelial cells, Epithelial cells, Hematopoietic stem and progenitors, Mast cells, Megakaryocytes
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Zola H et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiely-Liss A John Wiley & Sons Inc, Publication 2. Bachelet I, et al. 2006. J. Bio. Chem. 281:27190 3. Pende D, et al. 2006. Blood 107:2030 4. Bottino C, et al. 2003. J. Exp. Med. 198:557
Gene ID: 5819
UniProt: View information about CD112 on UniProt.org
Clone: TX31
Regulatory Status: RUO
Other Names: Poliovirus Receptor Related 2 Protein (PRR2), Nectin-2, Hve B
Isotype: Mouse IgG1, κ
Q: Do you guarantee that your antibodies are totally pathogen free?
A: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Q: Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
A: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Q: Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
A: No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.
Q: Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
A: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.