Biolegend, 338602, Purified anti-human CD132 (common γ chain) Antibody, 100μg

CD132 is a 64-70 kD type I transmembrane glycoprotein of the Ig superfamily, also known as common γ chain (γc), or IL-2 receptor γ subunit. It is expressed broadly on T- and B-lymphocytes, NK cells, monocytes, and granulocytes. CD132 is an essential component of cytokine receptors for IL-2, IL-4, IL-7, IL-9, IL-15 and IL-21. Ligand binding induces tyrosine phosphorylation and initiates signaling through a JAK/STAT pathway. CD132 mutation results in X-linked severe combined immune deficiency (XSCID).
100μg
Verified Reactivity: Human
Reported Reactivity: African Green, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Itano M, et al. 1996. J. Exp. Med. 178:389 Kondo M, et al. 1993. Science 262:1874
Product Citations: Chen Q, et al. 2015. Sci Rep. 15118:5. PubMed Laudanski K, et al. 2016. Med Sci Monit. 2463:22. PubMed
RRID: AB_1279081 (BioLegend Cat. No. 338602)
Structure: Type I transmembrane glycoprotein, Ig superfamily 64-70 kD
Distribution: T cells, B cells, NK, monocytes, granulocytes
Ligand/Receptor: Component of cytokine receptors for IL-2, IL-4, IL-7, IL-9, IL-15 and IL-21
Cell Type: B cells, Granulocytes, Monocytes, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Zola H, et al. eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiely-Liss A John Wiley & Sons Inc, Publication 2. Nakarai T, et al. 1994. J. Exp. Med. 180:241 3. Kawahara A, et al. 1995. Proc. Natl. Acad. Sci. USA. 92:8724 4. Habib T, et al. 2002. Biochemistry. 41:8725 5. Matthews DJ, et al. 1995. Blood 85:38
Gene ID: 3561
UniProt: View information about CD132 on UniProt.org
Clone: TUGh4
Regulatory Status: RUO
Workshop: VI C-89
Other Names: Common γ chain, γc, IL-2 receptor γ subunit
Isotype: Rat IgG2b, κ