Biolegend, 338608, APC anti-human CD132 (common γ chain) Antibody, 100tests

CD132 is a 64-70 kD type I transmembrane glycoprotein of the Ig superfamily, also known as common γ chain (γc), or IL-2 receptor γ subunit. It is expressed broadly on T- and B-lymphocytes, NK cells, monocytes, and granulocytes. CD132 is an essential component of cytokine receptors for IL-2, IL-4, IL-7, IL-9, IL-15 and IL-21. Ligand binding induces tyrosine phosphorylation and initiates signaling through a JAK/STAT pathway. CD132 mutation results in X-linked severe combined immune deficiency (XSCID).
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Rat
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application References(PubMed link indicates BioLegend citation): Itano M, et al. 1996. J. Exp. Med. 178:389 Kondo M, et al. 1993. Science 262:1874
Product Citations: Market M, et al. 2022. Int J Mol Sci. 23: . PubMed Wefers C, et al. 2018. Int J Mol Sci. 19. PubMed Rajawat YS, et al. 2021. Hum Gene Ther. 32:113. PubMed Market M, et al. 2020. Front Immunol. 1.127083333. PubMed Kumar A, et al. 2021. STAR Protoc. 2:100874. PubMed Matsubara Y, et al. 2014. Sci Rep. 4:5043. PubMed Massoud R, et al. 2015. Proc Natl Acad Sci U S A. 112: 11030-11035. PubMed Farhat AM, et al. 2021. Cell Reports. 35(4):109044. PubMed Hodge L, et al. 2012. Blood. 120:3774. PubMed Jiang J, et al. 2016. Sci Rep. 6: 32320. PubMed
RRID: AB_2123585 (BioLegend Cat. No. 338607) AB_2123584 (BioLegend Cat. No. 338608)
Structure: Type I transmembrane glycoprotein, Ig superfamily 64-70 kD
Distribution: T cells, B cells, NK, monocytes, granulocytes
Ligand/Receptor: Component of cytokine receptors for IL-2, IL-4, IL-7, IL-9, IL-15 and IL-21
Cell Type: B cells, Granulocytes, Monocytes, NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Zola H, et al. eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiely-Liss A John Wiley & Sons Inc, Publication 2. Nakarai T, et al. 1994. J. Exp. Med. 180:241 3. Kawahara A, et al. 1995. Proc. Natl. Acad. Sci. USA. 92:8724 4. Habib T, et al. 2002. Biochemistry. 41:8725 5. Matthews DJ, et al. 1995. Blood 85:38
Gene ID: 3561
UniProt: View information about CD132 on UniProt.org
Clone: TUGh4
Regulatory Status: RUO
Workshop: VI C-89
Other Names: Common γ chain, γc, IL-2 receptor γ subunit
Isotype: Rat IgG2b, κ