Biolegend, 338816, PE/Cyanine7 anti-human CD44 Antibody, 100tests

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from a low or mid level of intensity to high expression levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Normal human PBL
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Kishimoto T, et al. eds. 1997 Leucocyte Typing VI:White Cell Differentiation Antigen. Garland Publishing Inc.
Product Citations: Bersini S, et al. 2020. Life Sci Alliance. 3:e201900623. PubMed
RRID: AB_2716000 (BioLegend Cat. No. 338815) AB_2716001 (BioLegend Cat. No. 338816)
Structure: Variable splicing of CD44 gene generates many CD44 isoforms, 85 kD
Distribution: All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells
Function: Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor: Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type: Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area: Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Haynes BF, et al. 1991. Cancer Cells 3:347. 3. Goldstein LA, et al. 1989. Cell 56:1063. 4. Mikecz K, et al. 1995. Nat. Med. 1:558.
Gene ID: 960
UniProt: View information about CD44 on UniProt.org
Clone: BJ18
Regulatory Status: RUO
Workshop: VI A034
Other Names: Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype: Mouse IgG1, κ