Biolegend, 339002, Purified anti-human CD122 (IL-2Rβ) Antibody, 100μg

CD122 is a 70-75 kD type I transmembrane glycoprotein and member of the Ig superfamily. It is IL-2 receptor β chain also known as IL-2Rβ, which is also shared by the IL-15 receptor. CD122 is constitutively expressed by NK cells and at lower levels by a subset of T cells. Its expression is upregulated upon activation. The IL-2Rβ chain can combine with either the common γ subunit (γc, CD132) alone or with the γc subunit and the IL-2Rα subunit (CD25) to generate intermediate or high affinity IL-2 receptor complexes, respectively. CD122 expression levels can be upregulated by activation.
100μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: TL-Mor cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications include (for the relevant formats) include: immunoprecipitation, blocking of IL-2 binding to CD122, and partial inhibition of IL-2 induced cell proliferation.
Application References(PubMed link indicates BioLegend citation): Takeshita T, et al. 1989. J. Exp. Med. 169:1323.
Product Citations: Zhang H, et al. 2023. Nat Immunol. 24:96. PubMed
RRID: AB_1501059 (BioLegend Cat. No. 339002)
Structure: Ig superfamily, forms high affinity IL-2 receptor with CD25 and CD132 chains or intermediate affinity receptor with CD132 alone, 70-75 kD
Distribution: NK cells, T subset
Function: Critical component of IL-2 and IL-15 signaling
Ligand/Receptor: IL-2, IL-15
Cell Type: NK cells, T cells, Tregs
Biology Area: Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors
Antigen References: 1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication 2. Minami Y, et al. 1993. Annu. Rev. Immunol. 11:245. 3. Suzuki H, et al. 1995. Science 268:1472.
Gene ID: 3560
UniProt: View information about CD122 on UniProt.org
Clone: TU27
Regulatory Status: RUO
Workshop: V C050
Other Names: IL-2 Receptor β chain, IL-2Rβ
Isotype: Mouse IgG1, κ