Biolegend, 339711, APC anti-human CD300e (IREM-2, CMRF35-A5) Antibody, 25tests

IREM-2 (immune receptor expressed by myeloid cells 2), also known as CMRF35-A5, LMIR6, CD300Le or CD300e, is a 34 kD type I single transmembrane glycoprotein. It is a member of the CD300 family and of the Ig-superfamily. IREM-2 is expressed on monocyte and myeloid dendritic cells, but not on granulocytes and lymphoid dendritic cells. IREM-2 is an activating receptor through interaction with DAP12.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: IREM-IgG fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser: Red Laser (633 nm)
Application Notes: Additional reported applications include: immunoprecipitation and stimulation of TNF-a production by monocytes
Application References(PubMed link indicates BioLegend citation): Aguilar H, et al. 2004. J. Immunol. 173:6703 Gasiorowski RE, et al. 2013. Immunol Lett. 149:93. PubMed
RRID: AB_3097399 (BioLegend Cat. No. 339711) AB_3097399 (BioLegend Cat. No. 339712)
Structure: 34 kD, type I transmembrane glycoprotein, CD300 family, Ig superfamily
Distribution: Monocytes, macrophages, dendritic cells
Function: Activation of myeloid cells
Cell Type: Dendritic cells, Macrophages, Monocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Aguilar H, et al. 2004. J. Immunol. 173:6703
Gene ID: 342510
UniProt: View information about CD300e on UniProt.org
Clone: UP-H2
Regulatory Status: RUO
Other Names: CD300e, CMRF35-A5, LMIR6
Isotype: Mouse IgG1, κ