Biolegend, 339812, PE anti-BrdU Antibody, 100tests

BrdU is a uridine derivative and a structural analog of thymidine that can be incorporated into DNA during the S-phase of the cell cycle as a substitute for thymidine. Cells can be pulse-labeled with BrdU and analyzed with antibodies against BrdU to determine the proportion of cells in the S-phase of the cell cycle during a given interval.
100tests
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications include: immunohistochemical staining of formalin fixed paraffin embedded tissue sections.
Application References(PubMed link indicates BioLegend citation): Magaud J-P, et al. 1989. J. Histochem. Cytochem. 37:1517. Ejassi-Lassallette A, et al. 2011. 22:245. PubMed Ma L, et al. 2012 PNAS. 109:2090. PubMed.
Product Citations: Qi Z, et al. 2022. Nat Commun. 13:182. PubMed Zhang Y, et al. 2013. J Immunol. 900:4725. PubMed Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed Kenderes KJ, et al. 2018. Cell Rep. 24:824. PubMed Liu G, et al. 2019. Int J Biol Sci. 15:1383. PubMed Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed Kang DW, et al. 2020. J Pathol. 252:304. PubMed Frei A, et al. 2016. Nat Methods. 10.1038/nmeth.3742. PubMed Chandrasekaran AP, et al. 2021. Int J Mol Sci. 22:. PubMed Das S, et al. 2020. Cell Death Differ. 27:3004. PubMed Kolter J, et al. 2020. Immunity. 50(6):1482-1497. PubMed Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed Hosoya T, et al. 2009. J Exp Med. 206:2987. PubMed
RRID: AB_1626188 (BioLegend Cat. No. 339811) AB_1626186 (BioLegend Cat. No. 339812)
Structure: Uridine derivative that can be incorporated into DNA and substitute for thymidine residues.
Distribution: Cells can be pulsed labeled with BrdU which will be incorporated into DNA during the synthesis phase of the cell cycle.
Function: Antibody against BrdU can be used to identify cells undergoing DNA replication during the period of BrdU incorporation.
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Immunology
Molecular Family: Nuclear Markers
Antigen References: 1. Gratzner HG. 1982. Science 218:474. 2. Dolbeare F, et al. 1983. Proc. Natl. Acad. Sci. USA 80:5573. 3. Dolbeare F, et al. 1985. Cytometry 6:521.
Gene ID: NA
Clone: Bu20a
Regulatory Status: RUO
Other Names: Bromodeoxyuridine
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.