Biolegend, 340202, Purified anti-human CD307d (FcRL4) Antibody, 100μg

FcRL4 (Fc receptor-like 4) is a 57 kD single pass type I transmembrane protein that exhibits 4 Ig like C2 domains. Also known as ITRA1, IGFP2, and FcRH4, FcRL4 is predominately found on a subset of memory B cells. Activation of FcRL4 contributes to B cell receptor inhibition through activation of the tyrosine kinases SHP-1 and 2. FcRL4's nascent loci is 1q23.1; however, breakpoint mutations are associated with multiple myeloma and non-Hodgkins lymphoma. Increased FcRL4 expression on memory B cells has also been observed in HIV viremic patients. FcRL4 predominately binds aggregated IgA, binds weakly with IgG3 and IgG4, and does not bind IgG1 and IgG2.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: P815 cells transfected with FcRL4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested IP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Additional reported applications (with relevant formats) include: immunoprecipitation and blocking. mAb 413D12 is able to block the binding of FcRL4 to aggregated IgA, IgG3, and IgG4.
Application References(PubMed link indicates BioLegend citation): Sohn HW, et al. 2011. Blood. 118:6332. PubMed Wilson TJ, et al. 2012. J. Immunol. 188:4741. (Block)
Product Citations: Jourdan M, et al. 2017. PLoS One. 12(6):e0179793. PubMed
RRID: AB_2231789 (BioLegend Cat. No. 340202)
Structure: 57 kD, Fc receptor like, four Ig like domains
Distribution: Subset of memory B cells, some multiple myeloma and non-Hodgkins lymphoma
Interaction: Tyrosine phophorylated FcRL4 interacts with SHP-1, SHP-2 to inhibit the B cell receptor
Bioactivity: B-cell receptor inhibition
Cell Type: B cells
Biology Area: Immunology
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Moir S, et al. 2008. J. Exp. Med. 205:1797. 2. Ehrhardt GRA, et al. 2003. P. Natl. Acad. Sci. USA 100:13489. 3. Ehrhardt GRA, et al. 2005. J. Exp. Med. 202:783. 4. Polson AG, et al. 2006. J. Int. Immunol. 18:1363.
Gene ID: 83417
UniProt: View information about CD307d on UniProt.org
Clone: 413D12
Regulatory Status: RUO
Other Names: FcRL4 (Fc receptor-like 4), IRTA1, IGFP2
Isotype: Mouse IgG2b, κ