Biolegend, 340204, PE anti-human CD307d (FcRL4) Antibody, 100tests

FcRL4 (Fc receptor-like 4) is a 57 kD single pass type I transmembrane protein that exhibits 4 Ig like C2 domains. Also known as ITRA1, IGFP2, and FcRH4, FcRL4 is predominately found on a subset of memory B cells. Activation of FcRL4 contributes to B cell receptor inhibition through activation of the tyrosine kinases SHP-1 and 2. FcRL4's nascent loci is 1q23.1; however, breakpoint mutations are associated with multiple myeloma and non-Hodgkins lymphoma. Increased FcRL4 expression on memory B cells has also been observed in HIV viremic patients. FcRL4 predominately binds aggregated IgA, binds weakly with IgG3 and IgG4, and does not bind IgG1 and IgG2.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: P815 cells transfected with FcRL4
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications (with relevant formats) include: immunoprecipitation and blocking. mAb 413D12 is able to block the binding of FcRL4 to aggregated IgA, IgG3, and IgG4.
Application References(PubMed link indicates BioLegend citation): Sohn HW, et al. 2011. Blood. 118:6332. PubMed Wilson TJ, et al. 2012. J. Immunol. 188:4741. (Block)
Product Citations: Oliviero B, et al. 2011. J Hepatol. 55:53. PubMed SoRelle ED, et al. 2022. Front Immunol. 13:1001145. PubMed Li H, et al. 2016. J Immunol. 196: 4064 - 4074. PubMed Parmigiani A, et al. 2013. PLoS One. 13:79816. PubMed Sohn H, et al. 2011. Blood. 118:6332. PubMed Magri G et al. 2017. Immunity. 47(1):118-134 . PubMed Amara K, et al. 2017. J Autoimmun. 10.1016/j.jaut.2017.03.004. PubMed Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed Jenks SA et al. 2018. Immunity. 49(4):725-739 . PubMed Upasani V, et al. 2019. Front Immunol. 2.152777778. PubMed
RRID: AB_1575103 (BioLegend Cat. No. 340203) AB_2104578 (BioLegend Cat. No. 340204)
Structure: 57 kD, Fc receptor like, four Ig like domains
Distribution: Subset of memory B cells, some multiple myeloma and non-Hodgkins lymphoma
Interaction: Tyrosine phophorylated FcRL4 interacts with SHP-1, SHP-2 to inhibit the B cell receptor
Bioactivity: B-cell receptor inhibition
Cell Type: B cells
Biology Area: Immunology
Molecular Family: CD Molecules, Fc Receptors
Antigen References: 1. Moir S, et al. 2008. J. Exp. Med. 205:1797. 2. Ehrhardt GRA, et al. 2003. P. Natl. Acad. Sci. USA 100:13489. 3. Ehrhardt GRA, et al. 2005. J. Exp. Med. 202:783. 4. Polson AG, et al. 2006. J. Int. Immunol. 18:1363.
Gene ID: 83417
UniProt: View information about CD307d on UniProt.org
Clone: 413D12
Regulatory Status: RUO
Other Names: FcRL4 (Fc receptor-like 4), IRTA1, IGFP2
Isotype: Mouse IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.