Biolegend, 341304, PE anti-human CD158f (KIR2DL5) Antibody, 100tests

CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins with either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Some KIRs with long cytoplasmic domains contain ITIMs and posses inhibitory functions and others with short cytoplasmic region lack ITIM and have activation functions. 14 polymorphic KIR genes have been reported in humans. CD158f is a 60 kD glycoprotein with 2 Ig-like extracellular domains and long cytoplasmic domains. Its expression is highly polymorphic between individuals and mainly expressed on a subset of NK cells and a small population of T cells. HLA class I alleles are the ligands of CD158f.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human KIR2DL5-Ig fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Estefania E, et al. 2007. J. Immunol. 178:4402
Product Citations: Holder KA, et al. 2021. Clin Transl Immunology. 10:e1348. PubMed Chen M, et al. 2021. Cancers (Basel). 13:. PubMed Mou SQ, et al. 2021. Front Pharmacol. 12:648688. PubMed Cisneros E, et al. 2017. Front Immunol. 0.776388889. PubMed Samarani S, et al. 2020. Mediators Inflamm. 2020:6401969. PubMed
RRID: AB_1595545 (BioLegend Cat. No. 341303) AB_2130701 (BioLegend Cat. No. 341304)
Structure: Contains two Ig-like extracellular domains, 60 kD
Distribution: Subset of NK cells and a small subset of T cells, polymorphic expression
Function: Inhibits NK cell function, play a role in self-tolerance.
Ligand/Receptor: HLA alleles
Cell Type: NK cells, T cells
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Zola H, et al. eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiely-Liss A John Wiley & Sons Inc, Publication 2. Gonzalez A, et al. 2008. Tissue Antigens. 72:11
Gene ID: 57292
UniProt: View information about CD158f on UniProt.org
Clone: UP-R1
Regulatory Status: RUO
Other Names: KIR2DL5
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.