Biolegend, 341506, APC anti-human CCR10 Antibody, 100tests

CCR10, also known as GPR-2, is a G-protein-coupled seven transmembrane CC-chemokine receptor. It is the receptor of CCL27 (CTACK/ALP/ILC/ESkine) and CCL28 (MEC) and is expressed on a small subset of T memory cells, IgA-secreting cells, EBV-immortalized B cells, dermal microvascular endothelial cells and dermal fibroblasts. The interaction of CCR10 with its ligands plays a role in the regulation of T cell homing into cutaneous site and IgA-secreting cells migration.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Armenian Hamster
Immunogen: N-terminal peptide of human CCR10
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: It has been observed that clone 6588-5 can interact with some tandem-dye antibody conjugates during multi-color staining, potentially leading to unwanted staining. These dyes include PE/Cyanine7, PE/Cyanine5, PE/Dazzle™ 594, APC/Cyanine7, APC/Fire™ 750, and PerCP/Cyanine5.5. This interaction can be resolved by sequentially staining with the 6588-5 antibody first and then followed by other antibodies of interest.
Product Citations: Khanam A, et al. 2021. Front Immunol. 11:599648. PubMed Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed Sugita S, et al. 2013. J Immunol. 190:5799. PubMed
RRID: AB_2291025 (BioLegend Cat. No. 341505) AB_2071887 (BioLegend Cat. No. 341506)
Structure: G-protein-coupled seven transmembrane protein
Distribution: Small subsets of T memory cells and IgA-secreting cells, EBV-immortalized B cells, dermal microvascular endothelial cells and dermal fibroblasts
Function: T cell homing into cutaneous site, regulation of IgA-secreting cells migration
Ligand/Receptor: CCL27 (CTACK/ALP/ILC/ESkine), CCL28 (MEC)
Cell Type: B cells, Endothelial cells, Fibroblasts, T cells
Biology Area: Immunology
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Hudak S, et al. 2002. J. Immunol. 169:1189 2. Kunkel EJ, et al. 2003. J. Clin. Invest. 111:1001 3. Homey B, et al. 2002. Nature Medicine. 8:157 4. Nakayama T, et al. 2002. J. Virol. 76:3072
Gene ID: 2826
UniProt: View information about CCR10 on UniProt.org
Clone: 6588-5
Regulatory Status: RUO
Other Names: GPR-2, C-C chemokine receptor 10
Isotype: Armenian Hamster IgG
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.