Biolegend, 341604, PE anti-human CX3CR1 Antibody, 100tests

CX3CR1 is a G-protein-coupled seven-transmembrane chemokine receptor, also called GPR13 or V28. It is expressed on NK cells, T cell subset, monocytes/macrophages, dendritic cells, and some malignant epithelial cells. CX3CL1 (known also as fractalkine and neurotactin) is the ligand of CX3CR1. CX3CL1 is a unique transmembrane molecule with a CX3C-motif chemokine domain and a mucin-like stalk. CX3CL1 is expressed by activated-endothelial cells, neurons, and astrocytes. The interaction of CX3CR1 and its ligand mediatesfirm cell adhesion and migration.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CX3CR1-EGFP fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Nishimura M, et al. 2002. J. Immunol. 168:6173. Nanki T, et al. 2002. Arthritis Rheum. 46:2878. Kobayashi T, et al. 2007. Inflamm. Bowel Dis. 13:837. Beziat V, et al. 2011. J. Immunol. 186:6753. PubMed.
Product Citations: Adamo S, et al. 2023. Cell Host Microbe. 31:928. PubMed Feige L, et al. 2022. Viruses. 15: . PubMed Cannon J, et al. 2014. Physiol Rep. 2:12177. PubMed Weiskopf D, et al. 2015. Proc Natl Acad Sci U S A. 112: E4256-E4263. PubMed Morandi F, et al. 2014. J Immunol. 192:2634. PubMed Lewis SA, et al. 2021. Front Immunol. 12:724015. PubMed Izmirly AM, et al. 2022. PLoS Pathog. 18:e1009903. PubMed Sureshchandra S, et al. 2021. iScience. 24:102690. PubMed Buggert M, et al. 2020. Cell. 183(7):1946-1961.e15. PubMed Fraccarollo D, et al. 2021. eLife. 0.416666666666667. PubMed Guttikonda SR, et al. 2021. Nat Neurosci. 24:343. PubMed Agace W, et al. 2014. J Leukoc Biol. 95:531. PubMed Leers M,et al. 2017. Cytometry A.. 10.1002/cyto.a.23263. PubMed Yin S, et al. 2015. Sci Rep. 5: 14432. PubMed Collins PL et al. 2018. Cell. 176(1-2):348-360 . PubMed
RRID: AB_1595552 (BioLegend Cat. No. 341603) AB_1595456 (BioLegend Cat. No. 341604)
Structure: G-protein-coupled seven transmembrane receptor
Distribution: NK cells, T cell subset, monocytes/macrophages, dendritic cells
Function: cell adhesion and migration
Ligand/Receptor: CX3CL1 (Fractalkine, FKN, neurotactin)
Cell Type: Dendritic cells, Macrophages, Monocytes, NK cells, T cells
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family: Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Imai T, et al. 1997. Cell. 91:521. 2. Fong AM, et al. 1998. J. Exp. Med. 188:1413. 3. Auffray C, et al. 2009. J. Exp. Med. 206:595.
Gene ID: 1524
UniProt: View information about CX3CR1 on UniProt.org
Clone: 2A9-1
Regulatory Status: RUO
Other Names: V28, GPR13, Chemokine C-X3-C receptor 1
Isotype: Rat IgG2b, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.