Biolegend, 341620, Brilliant Violet 421™ anti-human CX3CR1 Antibody, 100tests

CX3CR1 is a G-protein-coupled seven-transmembrane chemokine receptor, also called GPR13 or V28. It is expressed on NK cells, T cell subset, monocytes/macrophages, dendritic cells, and some malignant epithelial cells. CX3CL1 (known also as fractalkine and neurotactin) is the ligand of CX3CR1. CX3CL1 is a unique transmembrane molecule with a CX3C-motif chemokine domain and a mucin-like stalk. CX3CL1 is expressed by activated-endothelial cells, neurons, and astrocytes. The interaction of CX3CR1 and its ligand mediatesfirm cell adhesion and migration.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: CX3CR1-EGFP fusion protein
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application References(PubMed link indicates BioLegend citation): Nishimura M, et al. 2002. J. Immunol. 168:6173. Nanki T, et al. 2002. Arthritis Rheum. 46:2878. Kobayashi T, et al. 2007. Inflamm. Bowel Dis. 13:837. Beziat V, et al. 2011. J. Immunol. 186:6753. PubMed.
Product Citations: Theodoraki MN, et al. 2023. Oncol Lett. 25:200. PubMed Asashima H, et al. 2023. Cell Rep. 42:111895. PubMed Crinier A, et al. 2018. Immunity. 49:971. PubMed Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed Medeiros-Furquim T, et al. 2022. Front Immunol. 13:678817. PubMed
RRID: AB_2687147 (BioLegend Cat. No. 341619) AB_2687148 (BioLegend Cat. No. 341620)
Structure: G-protein-coupled seven transmembrane receptor
Distribution: NK cells, T cell subset, monocytes/macrophages, dendritic cells
Function: cell adhesion and migration
Ligand/Receptor: CX3CL1 (Fractalkine, FKN, neurotactin)
Cell Type: Dendritic cells, Macrophages, Monocytes, NK cells, T cells
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Molecular Family: Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Imai T, et al. 1997. Cell. 91:521. 2. Fong AM, et al. 1998. J. Exp. Med. 188:1413. 3. Auffray C, et al. 2009. J. Exp. Med. 206:595.
Gene ID: 1524
UniProt: View information about CX3CR1 on UniProt.org
Clone: 2A9-1
Regulatory Status: RUO
Other Names: V28, GPR13, Chemokine C-X3-C receptor 1
Isotype: Rat IgG2b, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.
Q: Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
A: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.