Biolegend, 342403, PE anti-human C5L2 Antibody, 25tests

C5L2 (C5a receptor-like 2) is a seven-transmembrane orphan receptor, also known as GPR77, which shares significant homology to C5a receptor (CD88), but it is a decoy receptor without G-protein coupling. C5L2 is co-expressed with C5aR on many kinds of cells, such as neutrophils, immature dendritic cells, mast cells, macrophages, and astrocytes. C5L2 binds C5a with similar high affinity as C5aR and relatively higher affinity for C5a-des-Arg than with C5aR.
25tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: Additional reported applications include: Western blotting1,3 and blocking2,3. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team). Clone 1D9-M12 is known to block C5a binding to C5L2, but does not react with C5a receptor. It has been reported that this clone is not effective in immunohistochemistry of formalin-fixed paraffin-embedded sections1.
Application References(PubMed link indicates BioLegend citation): Fonseca MI, et al. 2013. J. Neuroinflammation. 10:25. (WB) PubMed Hao J, et al. 2013. PLoS One 8:e66305. (Block) Raby AC, et al. 2011. Eur. J. Immunol. 41:2741. (Block, WB)
Product Citations: Spaan A, et al. 2015. J Immunol. 195: 1034-1043. PubMed Derer S, et al. 2015. J Immunol. 195: 5077 - 5087. PubMed Schmidt T, et al. 2015. PLoS One. 10: 0132703. PubMed Rigotto G, et al. 2021. Int J Mol Sci. 22:. PubMed Su S et al. 2018. Cell. 172(4):841-856 . PubMed
RRID: AB_2113253 (BioLegend Cat. No. 342403) AB_2247831 (BioLegend Cat. No. 342404)
Structure: Seven transmembrane protein, decoy receptor, does not couple to G-proteins.
Distribution: Neutrophils, immature dendritic cells, mast cells, macrophages, and astrocytes.
Function: Negative regulation of C5a function.
Ligand/Receptor: C5a, C5a-des-Arg.
Cell Type: Astrocytes, Dendritic cells, Macrophages, Mast cells, Neutrophils
Biology Area: Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family: Cytokine/Chemokine Receptors
Antigen References: 1. Scola AM, et al. 2009. Mol. Immunol. 46:1149 2. Johswich K, et al. 2006. J. Bio. Chem. 281:39088 3. Gerad NP, et al. 2005. J. Bio. Chem. 280:39677 4. Okinaga S, et al. 2003. Biochemistry. 42:9406
Gene ID: 27202
UniProt: View information about C5L2 on UniProt.org
Clone: 1D9-M12
Regulatory Status: RUO
Other Names: GPR77, C5a receptor-like 2, C5a anaphylatoxin chemotactic receptor
Isotype: Mouse IgG2a, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.