Biolegend, 342752, Purified anti-human ACE2 Antibody, 100μg

Angiotensin-converting enzyme 2 (ACE2) is a type one transmembrane protein, which shares approximately 60% homology with ACE. The predicted human ACE2 protein sequence consists of 805 amino acids, including an N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE2 catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7 involved in vasoconstriction. ACE2 has been shown to be a functional receptor for the spike glycoprotein of the human coronavirus, HCoV-NL63, and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2. Based on recent single-cell RNA sequencing database, high ACE2 RNA expression was identified in the lung, esophagus, kidney, intestine, bile duct, heart, testis, endothelial cells, and vascular smooth muscle cells.
100μg
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
RRID: AB_3106182 (BioLegend Cat. No. 342752)
Structure: Type one transmemberance protein. Catalytic domain.
Distribution: Type II alveolar cells (AT2) of lung, esophagus upper and stratified epithelial cells, kidney proximal tubule cells, enterocytes in ileum and colon, cholangiocyte in bile duct, myocardial cells in heart, endothelium and vascular smooth muscle cells
Function: Catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7 involved in vasoconstriction
Cell Type: Epithelial cells
Biology Area: Cell Adhesion, COVID-19
Molecular Family: Adhesion Molecules
Antigen References: South AM, et al. 2020. Nat Rev Nephrol. 16:305-307. Hoffmann M, et al. 2020. Cell. 181:271-280. Sungnak W, et al. 2020. Nat Med. 26:681-687. Li W, et al. 2003. Nature. 426:450-4. Heurich A, et al. 2014. J Virol. 88:1293-307. Hamming I, et al. 2004. J Pathol. 203:631-7.
Gene ID: 59272
UniProt: View information about ACE2 on UniProt.org
Clone: 4G8C11
Regulatory Status: RUO
Other Names: Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACE-2, ACEH
Isotype: Mouse IgG1, κ