Biolegend, 342901, Purified anti-human TCR Vα24-Jα18 (iNKT cell) Antibody, 25μg

Encoded by the TCR Vα24-Jα18 germline configuration, Vα24-JαQ is expressed on a subset of NKT cells, namely invariant NKT (iNKT). Vα24-JαQ TCR interacts with the glycolipid loaded MHC class 1b molecule CD1d, inducing activation and subsequent cytokine production. iNKT cells have been implicated in immune regulation, tumor surveillance, and host response to pathogens. While iNKT cells occur at low frequency in the blood, assorted chemokines contribute to their tissue homing potential.
25μg
Verified Reactivity: Human
Reported Reactivity: Rhesus, Pigtailed Macaque, Sooty Mangabey
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The 6B11 antibody recognizes the invariant CDR3 region of TCR Va24-JaQ.
Application References(PubMed link indicates BioLegend citation): Rout N, et al. 2010. PLoS One 5:e9787. (FC)
Product Citations: Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed Nugent JL, et al. 2021. iScience. 24:103421. PubMed Kaufmann M, et al. 2021. Med. 2(3):296-312.e8. PubMed Speir M,et al. 2017. Sci Rep.. 10.1038/s41598-017-14690-5. PubMed Schwabenland M, et al. 2021. Immunity. . PubMed Melo AM, et al. 2020. Cancer Immunol Immunother. 69:969. PubMed Martin E, et al. 2020. JCI Insight. :5. PubMed
RRID: AB_2229301 (BioLegend Cat. No. 342902)
Structure: Invariant CDR3 region of TCR Vα24-JαQ
Distribution: Invariant NKT cells
Ligand/Receptor: Glycolipid loaded CD1d
Cell Type: NKT cells
Biology Area: Immunology
Molecular Family: TCRs
Antigen References: Thomas SY, et al. 2003. J. Immunol. 171:2571. Exley MA, et al. 2008. Eur. J. Immunol. 38:1756. Montoya CJ, et al. 2007. Immunology. 122:1. Gansuvd B, et al. 2003. J. Immunol. 171:2904.
Gene ID: 28659
UniProt: View information about TCR Valpha24-Jalpha18 on UniProt.org
Clone: 6B11
Regulatory Status: RUO
Other Names: TCR Vα24-Jα18, TCR Vα24-JαQ, invariant NKT cell, iNKT
Isotype: Mouse IgG1, κ