Biolegend, 342916, Brilliant Violet 421™ anti-human TCR Vα24-Jα18 (iNKT cell) Antibody, 100tests

Encoded by the TCR Vα24-Jα18 germline configuration, Vα24-JαQ is expressed on a subset of NKT cells, namely invariant NKT (iNKT). Vα24-JαQ TCR interacts with the glycolipid loaded MHC class 1b molecule CD1d, inducing activation and subsequent cytokine production. iNKT cells have been implicated in immune regulation, tumor surveillance, and host response to pathogens. While iNKT cells occur at low frequency in the blood, assorted chemokines contribute to their tissue homing potential.
100tests
Verified Reactivity: Human
Reported Reactivity: Rhesus, Pigtailed Macaque, Sooty Mangabey
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd. Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: The 6B11 antibody recognizes the invariant CDR3 region of TCR Va24-JaQ.
Application References(PubMed link indicates BioLegend citation): Rout N, et al. 2010. PLoS One 5:e9787. (FC)
Product Citations: Ollé Hurtado M, et al. 2019. PLoS One. 14:e0216373. PubMed
RRID: AB_2564004 (BioLegend Cat. No. 342915) AB_2721323 (BioLegend Cat. No. 342916)
Structure: Invariant CDR3 region of TCR Vα24-JαQ
Distribution: Invariant NKT cells
Ligand/Receptor: Glycolipid loaded CD1d
Cell Type: NKT cells
Biology Area: Immunology
Molecular Family: TCRs
Antigen References: Thomas SY, et al. 2003. J. Immunol. 171:2571. Exley MA, et al. 2008. Eur. J. Immunol. 38:1756. Montoya CJ, et al. 2007. Immunology. 122:1. Gansuvd B, et al. 2003. J. Immunol. 171:2904.
Gene ID: 28659
UniProt: View information about TCR Valpha24-Jalpha18 on UniProt.org
Clone: 6B11
Regulatory Status: RUO
Other Names: TCR Vα24-Jα18, TCR Vα24-JαQ, invariant NKT cell, iNKT
Isotype: Mouse IgG1, κ
Q: What is the F/P ratio range of our BV421™ format antibody reagents?
A: It is lot-specific. On average it ranges between 2-4.