Biolegend, 343201, Purified anti-human CD43 Antibody, 25μg

CD43, also known as Ly-48, leukosialin, sialophorin, leukocyte sialoglycoprotein, and W3/13, is a large single chain, type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors.
25μg
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: MV4-11 cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality testedIP - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application References(PubMed link indicates BioLegend citation): Rosenkranz AR, et al. 1993. Immunology. 80:431 Skubitz KM, et al. 1998. J. Leukoc. Biol. 64:800
Product Citations: Kirolos SA, et al. 2022. J Leukoc Biol. 112:1399. PubMed Xu C, et al. 2022. iScience. 25:105123. PubMed Eccles JD, et al. 2020. Cell Rep. 30:351. PubMed Donnelly C, et al. 2018. Sci Rep. 0.625. PubMed Crawford LB, et al. 2021. J Virol. 95:. PubMed
RRID: AB_1659198 (BioLegend Cat. No. 343202)
Structure: Single chain, type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 95-130 kD.
Distribution: Most hematopoietic cells, including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, activated B cells, and hemotopoietic progenitor cells.
Function: Plays dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, inducing apoptosis of T cells and hematopoietic progenitors.
Ligand/Receptor: CD54, Siglec-1
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Neil Barclay A, et al. 1997. The Leucocyte Antigen Facts Book:Second Edition. Page 238 2. Killeen A, et al. 1987. EMBO J. 6:4029. 3. Thurman EC, et al. 1998. Intl. Immunol. 10:691. 4. Onami TM, et al. 2002. J. Immunol. 168:6022. 5. Tong J, et al. 2004. J. Exp. Med. 199:1277. 6. Jones AT, et al. 1994. J. Immunol. 153:3426.
Gene ID: 6693
UniProt: View information about CD43 on UniProt.org
Clone: CD43-10G7
Regulatory Status: RUO
Other Names: Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein
Isotype: Mouse IgG1, κ