Biolegend, 343204, PE anti-human CD43 Antibody, 100tests

CD43, also known as Ly-48, leukosialin, sialophorin, leukocyte sialoglycoprotein, and W3/13, is a large single chain, type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors.
100tests
Verified Reactivity: Human
Reported Reactivity: African Green, Baboon, Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: MV4-11 cell line
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References(PubMed link indicates BioLegend citation): Rosenkranz AR, et al. 1993. Immunology. 80:431 Skubitz KM, et al. 1998. J. Leukoc. Biol. 64:800
Product Citations: Jenkins E, et al. 2023. Nat Commun. 14:1611. PubMed Stavish D, et al. 2020. Nat Commun. 3.922916667. PubMed Xu Y, et al. 2021. Stem Cell Res Ther. 12:112. PubMed Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed Martin RM et al. 2019. Cell Stem Cell. 24(5):821-828 . PubMed
RRID: AB_1659201 (BioLegend Cat. No. 343203) AB_2255209 (BioLegend Cat. No. 343204)
Structure: Single chain, type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 95-130 kD.
Distribution: Most hematopoietic cells, including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, activated B cells, and hemotopoietic progenitor cells.
Function: Plays dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, inducing apoptosis of T cells and hematopoietic progenitors.
Ligand/Receptor: CD54, Siglec-1
Biology Area: Immunology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Neil Barclay A, et al. 1997. The Leucocyte Antigen Facts Book:Second Edition. Page 238 2. Killeen A, et al. 1987. EMBO J. 6:4029. 3. Thurman EC, et al. 1998. Intl. Immunol. 10:691. 4. Onami TM, et al. 2002. J. Immunol. 168:6022. 5. Tong J, et al. 2004. J. Exp. Med. 199:1277. 6. Jones AT, et al. 1994. J. Immunol. 153:3426.
Gene ID: 6693
UniProt: View information about CD43 on UniProt.org
Clone: CD43-10G7
Regulatory Status: RUO
Other Names: Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein
Isotype: Mouse IgG1, κ
Q: What type of PE do you use in your conjugates?
A: We use R-PE in our conjugates.