Biolegend, 343316, PerCP/Cyanine5.5 anti-human HLA-A2 Antibody, 100tests

HLA-A2 is most common in Northern Asia and North America populations. MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8 + T cells, playing an important role in cell-mediated immune responses and tumor surveillance.
100tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Papain solubilized HLA-A2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: The BB7.2 antibody recognizes human leukocyte antigen (HLA) A2 which is a subset of MHC-class I molecules encoded by A*02 alleles. Additional reported applications (for the relevant formats) include: immunoprecipitation3.
Application References(PubMed link indicates BioLegend citation): Brodsky FM, et al. 1979. Immunol. Rev. 47:3. Parham P and Brodsky FM. et al. 1981. Hum. Immunol. 3:277. Lubben NB, et al. 2007. Mol Biol Cell. 18:3351. (IP)
Product Citations: Zhu F, et al. 2024. J Immunother Cancer. :12. PubMed
RRID: AB_2561572 (BioLegend Cat. No. 343315) AB_2561573 (BioLegend Cat. No. 343316)
Structure: Ig superfamily
Distribution: A*02 individuals leukocytes
Function: Antigen presentation
Ligand/Receptor: CD8, CD3/TCR
Cell Type: Leukocytes
Biology Area: Immunology
Molecular Family: MHC Antigens
Gene ID: 3105
UniProt: View information about HLA-A2 on UniProt.org
Clone: BB7.2
Regulatory Status: RUO
Other Names: MHC-class I
Isotype: Mouse IgG2b, κ
Q: How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?
A: PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.