Biolegend, 343323, Brilliant Violet 650™ anti-human HLA-A2 Antibody, 25tests

HLA-A2 is most common in Northern Asia and North America populations. MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells. MHC class I molecules are involved in presentation of antigens to CD8 + T cells, playing an important role in cell-mediated immune responses and tumor surveillance.
25tests
Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Papain solubilized HLA-A2
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation: The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.Learn more about Brilliant Violet™. This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser: Violet Laser (405 nm)
Application Notes: The BB7.2 antibody recognizes human leukocyte antigen (HLA) A2 which is a subset of MHC-class I molecules encoded by A*02 alleles. Additional reported applications (for the relevant formats) include: immunoprecipitation3.
Application References(PubMed link indicates BioLegend citation): Brodsky FM, et al. 1979. Immunol. Rev. 47:3. Parham P and Brodsky FM. et al. 1981. Hum. Immunol. 3:277. Lubben NB, et al. 2007. Mol Biol Cell. 18:3351. (IP)
Product Citations: Ning J, et al. 2022. Cancer Immunol Immunother. 71:1863. PubMed Rosato PC, et al. 2019. Nat Commun. 10:567. PubMed
RRID: AB_2687268 (BioLegend Cat. No. 343323) AB_2687269 (BioLegend Cat. No. 343324)
Structure: Ig superfamily
Distribution: A*02 individuals leukocytes
Function: Antigen presentation
Ligand/Receptor: CD8, CD3/TCR
Cell Type: Leukocytes
Biology Area: Immunology
Molecular Family: MHC Antigens
Gene ID: 3105
UniProt: View information about HLA-A2 on UniProt.org
Clone: BB7.2
Regulatory Status: RUO
Other Names: MHC-class I
Isotype: Mouse IgG2b, κ