Biolegend, 343512, Pacific Blue™ anti-human CD34 Antibody, 100μg

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.
100μg
Verified Reactivity: Human
Reported Reactivity: Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. * Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome. Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.View full statement regarding label licenses
Excitation Laser: Violet Laser (405 nm)
Application Notes: The 581 antibody recognizes the class III group epitope which is resistant to sialidase/glycolyprotease and chymopapain treatment. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections5 and immunofluorescence6.
Application References(PubMed link indicates BioLegend citation): Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigen. New York:Oxford University Press. Felschow DM, et al. 2001. Blood 97:3768. Rudin CE, et al. 1997. Br. J. Haematol. 97:488. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Skowasch D, et al. 2003. Cardiovasc Res. 60:684. (IHC) Umland O, et al. 2003. J. Histochem. Cytochem. 51:977. (IF)
Product Citations: Xu H, et al. 2019. Cell Stem Cell. 24:566. PubMed Scala S, et al. 2023. Nat Commun. 14:3068. PubMed Biancon G, et al. 2022. Mol Cell. 82:1107. PubMed Qiu J, et al. 2020. Cell Rep. 33:108465. PubMed Mitchell K, et al. 2018. J Exp Med. 215:1709. PubMed Queckborner S, et al. 2020. Stem Cell Res Ther. 11:15. PubMed Wu Y, et al. 2019. Nat Med. 25:776. PubMed Bhatwadekar A, et al. 2015. Diabetes. 64: 4226 - 4237. PubMed Van Egeren D, et al. 2021. Cell Stem Cell. 28(3):514-523.e9. PubMed Osorio FG et al. 2018. Cell reports. 25(9):2308-2316 . PubMed Velázquez–Avila M, et al. 2019. Leukemia. 33:1337. PubMed Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed Zeng J, et al. 2020. Nat Med. 26:535. PubMed Pellin D, et al. 2019. Nat Commun. 10:2395. PubMed Bhatwadekar A, et al. 2010. Diabetes. 59:2010. PubMed Roerden M, et al. 2021. Cancers. 13(11):. PubMed Pribyl M, et al. 2020. Mol Oncol. 2.252083333. PubMed Ueda K, et al. 2021. Cancer Cell. 39(4):529-547.e7. PubMed Rai S, et al. 2022. Nat Commun. 13:5346. PubMed Jarajapu Y, et al. 2013. Diabetes. 62:1258. PubMed Yang C, et al. 2019. Nat Commun. 10:3931. PubMed
RRID: AB_1877198 (BioLegend Cat. No. 343511) AB_1877197 (BioLegend Cat. No. 343512)
Structure: 105-120 kD single chain mucin-like glycoprotein
Distribution: Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts
Function: Cell adhesion
Ligand/Receptor: L-selectin, E-selectin
Cell Type: Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Krause DS, et al. 1996. Blood 87:1. 2. Puri KD, et al. 1995. J. Cell Biol. 131:261. 3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey.
Gene ID: 947
UniProt: View information about CD34 on UniProt.org
Clone: 581
Regulatory Status: RUO
Workshop: V MA27
Other Names: Gp105-120, My10
Isotype: Mouse IgG1, κ