Biolegend, 343514, APC/Cyanine7 anti-human CD34 Antibody, 100tests

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.
100tests
Verified Reactivity: Human
Reported Reactivity: Cynomolgus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Red Laser (633 nm)
Application Notes: The 581 antibody recognizes the class III group epitope which is resistant to sialidase/glycolyprotease and chymopapain treatment. Additional reported applications (for the relevant formats) include: immunohistochemical staining of paraffin-embedded tissue sections5 and immunofluorescence6.
Application References(PubMed link indicates BioLegend citation): Schlossman SF, et al. 1995. Leukocyte Typing V:White Cell Differentiation Antigen. New York:Oxford University Press. Felschow DM, et al. 2001. Blood 97:3768. Rudin CE, et al. 1997. Br. J. Haematol. 97:488. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC) Skowasch D, et al. 2003. Cardiovasc Res. 60:684. (IHC) Umland O, et al. 2003. J. Histochem. Cytochem. 51:977. (IF)
Product Citations: Kfoury Y, et al. 2021. Cancer Cell. 39:1464. PubMed Dickerson KM, et al. 2022. Blood Cancer Discov. 3:240. PubMed Baudet A, et al. 2022. Bio Protoc. 12:e4353. PubMed Takayama N, et al. 2020. Cell Stem Cell. 28(3):488-501.e10. PubMed Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed Kokkinopoulos I, et al. 2021. Sci Rep. 11:15759. PubMed Shukla S, et al. 2021. Nat Chem Biol. 17:784. PubMed Mori Y, et al. 2015. Proc Natl Acad Sci U S A. 112: 9638-9643. PubMed Nguyen MAT, et al. 2020. Immunohorizons. 0.465277778. PubMed Lal A, et al. 2021. Nat Commun. 1507:12. PubMed Bigley V, et al. 2011. J Exp Med. 208:227. PubMed Montefiori LE, et al. 2021. Cancer Discov. 11:2846. PubMed Zhou X, et al. 2017. J Vasc Surg. 65:1161. PubMed Olesinski EA, et al. 2021. STAR Protocols. 2(2):100461. PubMed Dorrell C, et al. 2016. Nat Commun. 7:11756. PubMed Hofmann M, et al. 2012. Leukemia. 26:1288. PubMed Kohn L, et al. 2014. J Immunol. 192:5050. PubMed Bigley V, et al. 2015. J Leukoc Biol. 97:627. PubMed Isobe T, et al. 2022. Nat Commun. 13:4501. PubMed Lee J, et al. 2015. J Exp Med. 212:385. PubMed Breton G, et al. 2015. J Exp Med. 212:401. PubMed Berent-Maoz B, et al. 2015. PLoS One. 10:125414. PubMed Gupta R, et al. 2020. Cell Stem Cell. 26:527. PubMed
RRID: AB_1877169 (BioLegend Cat. No. 343513) AB_1877168 (BioLegend Cat. No. 343514)
Structure: 105-120 kD single chain mucin-like glycoprotein
Distribution: Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts
Function: Cell adhesion
Ligand/Receptor: L-selectin, E-selectin
Cell Type: Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family: CD Molecules
Antigen References: 1. Krause DS, et al. 1996. Blood 87:1. 2. Puri KD, et al. 1995. J. Cell Biol. 131:261. 3. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. John Wiley & Sons Inc, Hoboken New Jersey.
Gene ID: 947
UniProt: View information about CD34 on UniProt.org
Clone: 581
Regulatory Status: RUO
Workshop: V MA27
Other Names: Gp105-120, My10
Isotype: Mouse IgG1, κ