Biolegend, 343601, Purified anti-human CD34 Antibody, 25μg

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glyco-phosphoprotein with an approximate molecular weight of 105-120 kD. It is selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells. Based on different sensitivities, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.
25μg
Verified Reactivity: Human
Reported Reactivity: Cynomolgus, Rhesus
Antibody Type: Monoclonal
Host Species: Mouse
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: The 561 antibody recognizes a class III group epitope, which is resistant to sialidase/glycolyprotease and chymopapain treatment.
Application References(PubMed link indicates BioLegend citation): Croockewit AJ, et al. 1998. Scand. J. Immunol. 47:82. Rosenzweig M, et al. 2001. J. Med. Primatol. 30:36.
Product Citations: Xu C, et al. 2022. iScience. 25:105123. PubMed Alcántara‐Hernández M et al. 2017. Immunity. 47(6):1037-1050 . PubMed Ortiz AM, et al. 2018. Nat Med. 24:1313. PubMed Alcántara-Hernández M, et al. 2021. Nat Protoc. 16:4855. PubMed Davidov T, et al. 2021. Int J Mol Sci. 22:. PubMed Ardain A, et al. 2019. Nature. 570:528. PubMed Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed Ghosh S, et al. 2019. Commun Biol. 0.325. PubMed Leylek R, et al. 2020. Cell Rep. 32:108180. PubMed
RRID: AB_1732014 (BioLegend Cat. No. 343602)
Structure: 105-120 kD single chain mucin-like glycoprotein
Distribution: Hematopoietic stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic fibroblasts
Function: Cell adhesion
Ligand/Receptor: L-selecin, E-selectin
Cell Type: Endothelial cells, Fibroblasts, Hematopoietic stem and progenitors
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience, Stem Cells
Molecular Family: CD Molecules
Gene ID: 947
UniProt: View information about CD34 on UniProt.org
Clone: 561
Regulatory Status: RUO
Other Names: Gp105-120, My10, Mucosialin
Isotype: Mouse IgG2a, κ